Elsevier

Gene

Volume 190, Issue 1, 1997, Pages 99-104
Gene

A novel MAP-kinase kinase from Candida albicans

https://doi.org/10.1016/S0378-1119(96)00758-5Get rights and content

Abstract

A putative MAP-kinase kinase-encoding gene, CaSTE7, was isolated from Candida albicans by complementation of ste7 and stell mutants of the pheromone signal-transduction pathway of Saccharomyces cerevisiae. The nucleotide (nt) sequence revealed an ORF of 1767 nt encoding a putative protein of 589 amino acids (aa). CaSTE7 has a strong homology with MAP-kinase kinase STE7 of S. cerevisiae, the kinase domain having 45% homology with that of STE7. The deduced aa sequence contained all eleven consensus kinase subdomains found in MAP-kinase kinases. It can suppress the mating defect of ste5, stell, ste7, and fus3 kssl double mutants, but it cannot bypass the ste12 mutation. CaSTE7 behaves as a hyperactive allele of STE7, suppressing the mating defects of the pheromone signal-transduction pathway by constitutively stimulating STE12, and hence STE12-dependent processes.

References (30)

  • K. Choi et al.

    Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae

    Cell

    (1994)
  • M. Company et al.

    Identification of a Tyl regulatory sequence responsive to STE7 and STE12

    Mol. Cell. Biol.

    (1988)
  • A. Datta et al.

    Current trends in Candida albicans research

    Adv. Microb. Physiol.

    (1989)
  • E.A. Elion et al.

    FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction

  • G.I. Evan et al.

    Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product

    Mol. Cell. Biol.

    (1985)
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