Quantitative assessment of human erythrocyte membrane solubilization by Triton X-100

doi:10.1016/S0301-4622(02)00043-1

Biophysical Chemistry

Volume 97, Issue 1, 23 May 2002, Pages 1-5

https://doi.org/10.1016/S0301-4622(02)00043-1 Get rights and content

Abstract

We report here a quantitative analysis of the interaction of the non-ionic surfactant Triton X-100 with human erythrocyte membranes. By applying a classical treatment for the interpretation of the action of surface active compounds to the hemolytic curves, we could calculate parameters such as R_e—the effective surfactant/lipid molar ratio for erythrocyte membrane saturation (R_e^sat) and total lysis (R_e^sol)—and K_b, the binding constant of Triton X-100 to human erythrocyte membranes. The K_b (5900 M⁻¹) and R_e (1.58 and 2.14) values presented here are in good agreement with literature data for Triton X-100 solubilization of model phospholipid membranes.

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Acknowledgements

Grants from Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES) and Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP, Proc. 01/03632-0) are gratefully acknowledged.

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LetterQuantitative assessment of human erythrocyte membrane solubilization by Triton X-100

Abstract

Section snippets

Acknowledgements

Int. J. Pharm.

FEBS Lett.

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Chem.-Biol. Interact.

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biophys. J.

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biophys. Chem.

Arch. Biochim. Biophys.

Letter
Quantitative assessment of human erythrocyte membrane solubilization by Triton X-100