Kir 4.1 channel expression in neuroblastoma×glioma hybrid NG108-15 cell line

doi:10.1016/S0165-3806(99)00015-2

Developmental Brain Research

Volume 114, Issue 1, 12 April 1999, Pages 127-134

https://doi.org/10.1016/S0165-3806(99)00015-2 Get rights and content

Abstract

To study a possible involvement of inwardly rectifying K⁺ 4.1 (K_ir 4.1) channels in neural cell development, RT-PCR, immunocytochemistry and whole-cell patch-clamp techniques were used to assess expression of K_ir 4.1 channels in proliferating and differentiated NG108-15 cells. RT-PCR revealed co-expression of K_ir 4.1 and rat ether-a-go-go-related gene (R-ERG) mRNAs in both proliferating and differentiated cells. The relative K_ir 4.1 mRNA concentration increased markedly as cells progressed from undifferentiated to differentiated cells. K_ir 4.1-immunoreactivity was barely detectable in undifferentiated cells, but clearly detected in differentiated cells, indicating that K_ir 4.1 gene and protein expressions are developmentally regulated. However, corresponding K_ir 4.1 current could not be detected in differentiated cells using whole-cell patch-clamp recording. The `silent' channel/receptor, often found in tumor cells, may carry genetic defects, which prevent functional expression of the channel. NG108-15 may serve as unique model for studying the relationship between the expression of an ion channel gene and the electrophysiological phenotype it encodes.

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Acknowledgements

The authors gratefully thank Dr. Kenton Swartz of NINDS, NIH for his comments. The opinions and assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Department of the Navy.

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Short communicationKir 4.1 channel expression in neuroblastoma×glioma hybrid NG108-15 cell line

Abstract

Section snippets

Acknowledgements

Biochemical and Biophysical Research Communications

Neuron

Int. Rev. Cytol.

Dev. Brain Res.

Integrin-mediated neurite outgrowth in neuroblastoma cells depends on the activation of potassium channels

J. Cell Biol.

A novel inward-rectifying K+ current with a cell-cycle dependence governs the resting potential of neuroblastoma cells

J. Physiol. (Lond.)

HERG- and IRK-like inward rectifier currents are sequentially expressed during neuronal development of neural crest cells and their derivatives

Eur. J. Neurosci.

herg encodes a K+ current highly conserved in tumors of different histogenesis: a selective advantage for cancer cells?

Cancer Res.

Immunological detection of glutamate receptor subtypes in human central nervous system

Ann. Neurol.

Cloning and expression of a family of inward rectifier potassium channels

Recept Channels