Chapter 24 Difficulties and pitfalls in analysis of apoptosis

doi:10.1016/S0091-679X(01)63028-0

Methods in Cell Biology

Volume 63, Part A, 2001, Pages 527-546

https://doi.org/10.1016/S0091-679X(01)63028-0 Get rights and content

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Flow cytometry has become the preferred methodology for analysis of apoptosis both in the research environment as well as in clinical settings. This chapter discusses the most common errors in measurement of apoptosis as well as the frequent mistakes in the analysis and interpretation of the data. There are certain issues associated with the inappropriate use of flow cytometry in the analysis of apoptosis. The common pitfalls and inappropriate uses of the methodology are apparent. Some of these problems are generic to most of the methods: they generally pertain to data interpretation, in particular how the frequency of apoptotic cells (apoptotic index) relates to the incidence of cell death in cultures or in tissue. Other problems are specific to particular methods or cell systems.

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Chapter 24 Difficulties and pitfalls in analysis of apoptosis

Publisher Summary

Exp. Cell Res.

Exp. Cell Res.

Anal. Biochem.

Exp. Cell Res.

Exp. Cell Res.

Blood

Exp. Cell Res.

Exp. Cell Res.

Exp. Cell Res.

Cell

Exp. Cell Res.

Cell

Apoptosis: The role of endonuclease

Am. J. Pathol.

Laser scanning cytometry distinguishes lymphocytes, monocytes and granulocytes by differences in their chromatin structure

Cytometry

Cell cycle effects and induction of apoptosis caused by infection with human granulocytic Ehrlichiosis pathogen measured by laser scanning cytometry

Cytometry

High affinity of binding of fluorescein isothiocynate to eosinophils detected by laser scanning cytometry: A potential source of error in analysis of blood samples utilizing fluorescein-conjugated reagents in flow cytometry

Cytometry

Analysis of apoptosis by laser scanning cytometry

Cytometry

Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation

Biochem. J.

Internucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis

Int. J. Radiat. Biol.

Measurements of cell death by flow cytometry

Assay of cell cycle kinetics by multivariate flow cytometry

Features of apoptotic cells measured by flow cytometry

Cytometry

Assays of cell viability Discrimination of cells dying by apoptosis

Cytometry in cell necrobiology: Analysis of apoptosis and accidental cell death (necrosis)

Cytometry

Critical aspects in the analysis of apoptosis

Hum. Cell

Methods for analysis of apoptosis by flow cytometry