Chapter 24 Difficulties and pitfalls in analysis of apoptosis
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2017, ChemosphereCitation Excerpt :Additionally, NPs may interact with marker molecules or chemical agents, resulting in problems when conducting common toxicity tests (Kroll et al., 2012; Karlsson et al., 2013; Guadagnini et al., 2015). Investigation of apoptosis via AnnexinV/propidium-iodide staining has returned false-positive results in situations where NPs were able to bind propidium iodide and be taken up by intact cells (Darzynkiewicz et al., 2001; Shukla et al., 2005). NPs also interfere with enzyme-linked immunosorbent assays owing to the ability of NPs to adsorb cytokines (Monteiro-Riviere and Inman, 2006; Veranth et al., 2007).
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