Interleukin-10 regulation in normal subjects and patients with asthma☆,☆☆,★,★★
Section snippets
Subjects
Assays were performed on cohorts of volunteers with asthma and normal control subjects (Table I, Table II). Normal volunteers were individuals who lacked a medical history consistent with atopic disease including asthma, allergic rhinitis, or eczema. Skin prick test responses to a Colorado allergen panel were negative, and at the time of study volunteers’ spirometry was normal. The asthmatic subjects met the criteria of the American Thoracic Society–American College of Chest Physicians for
IL-10 and IL-1β protein in BAL fluid
IL-10 protein concentration in BAL fluid was measured by an ELISA. Patient characteristics are summarized in Table I, Table II. IL-10 was readily measured in the BAL fluid of all of the normal subjects (130 ± 61 pg/ml; range, 41 to 211 pg/ml; n = 8). In contrast, only two of eight patients with asthma demonstrated IL-10 in the BAL fluid above the sensitivity of the ELISA, and even in these subjects, the concentration was lower than that observed in the normal subjects (9 ± 18 pg/ml; p < 0.01
DISCUSSION
Immune responses generated by cytokines are essential to the development of asthma. T lymphocyte–derived cytokines are required for the development of several phenotypic markers of asthma including IgE dysregulation (IL-4 and IL-1318, 19, 20), eosinophilia (IL-5, granulocyte-macrophage colony-stimulating factor, and IL-321, 22 ), mast cell proliferation (IL-3, IL-9, stem cell factor23, 24, 25), and enhanced histamine release (IL-3, RANTES, and monocyte chemotactic and activating factor26).
References (50)
- et al.
Modified immunological status of anti-IL-10 treated mice
Cell Immunol
(1993) - et al.
Cytokines in symptomatic asthma airways
J ALLERGY CLIN IMMUNOL
(1992) - et al.
Expression of the potent inflammatory cytokines, granulocyte-macrophage-colony-stimulating factor and interleukin-6 and interleukin-8, in bronchial epithelial cells of patients with asthma
J ALLERGY CLIN IMMUNOL
(1992) - et al.
Activation of CD4+ T cells, increased TH2-type cytokine mRNA expression, and eosinophil recruitment in bronchoalveolar lavage after allergen inhalation challenge in patients with atopic asthma
J ALLERGY CLIN IMMUNOL
(1993) - et al.
Glucocorticoid effects on the production and action of immune cytokines
J Steroid Biochem
(1988) - et al.
Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones
J Exp Med
(1989) - et al.
Direct effects of IL-10 on subsets of human CD4+ T cell clones and resting T cells: specific inhibition of IL-2 production and proliferation
J Immunol
(1993) - et al.
Human IL-10 is produced by both type 1 helper (Th1) and type 2 helper (Th2) T cell clones and inhibits their antigen-specific proliferation and cytokine production
J Immunol
(1993) - et al.
IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells
J Immunol
(1992) - et al.
IL-10 inhibits cytokine production by activated macrophages
J Immunol
(1991)
IL-10, T lymphocyte inhibitor of human blood cell production of IL-1 and tumor necrosis factor
J Immunol
Interleukin 10 (IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes
J Exp Med
Predominant role of tumor necrosis factor-α in human monocyte IL-10 synthesis
J Immunol
Macrophage deactivation by interleukin 10
J Exp Med
Interleukin 10 (IL-10) inhibits human lymphocyte interferon-γ production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells
J Exp Med
Differential effects of interleukin-4 and -10 on interleukin-2 induced interferon-γ synthesis and lymphokine-activated killer activity. Int
Immunol
Inhibition of Langerhans cell antigen-presenting function by IL-10: a role for IL-10 in induction of tolerance
J Immunol
Interleukin 10 inhibits lipopolysaccharide-induced survival and cytokine production by human peripheral blood eosinophils
J Exp Med
IL-10 and viral IL-10 prevent IL-4-induced IgE synthesis by inhibiting the accessory cell function of monocytes
J Immunol
Definition and classification of chronic bronchitis, asthma, and pulmonary emphysema
Am Rev Respir Dis
Airways inflammation in nocturnal asthma
Am Rev Respir Dis
Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples
Immunol Rev
B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells
J Immunol
IL4 is an essential factor for IgE synthesis induced in vitro by human T cell clones and their supernatants
J Immunol
Interleukin 13 induces interleukin 4-independent IgG4 and IgE synthesis and CD23 expression by human B cells
Proc Natl Acad Sci USA
Cited by (0)
- ☆
From the National Jewish Center for Immunology and Respiratory Medicine, University of Colorado Health Sciences Center, Denver.
- ☆☆
Supported by National Institutes of Health grants AI32244, and HL36577.
- ★
Reprint requests: Larry Borish, MD, Department of Medicine, National Center for Immunology and Respiratory Medicine, 1400 Jackson St., Denver, CO 80206.
- ★★
0091-6749/96 $5.00 + 0 1/1/68123