Adult urologyIncreased expression of PSA mRNA during brachytherapy in peripheral blood of patients with prostate cancer
Section snippets
Patients
Twenty-five patients undergoing brachytherapy for prostate cancer were recruited for this study with informed consent approved by University of Kentucky Institutional Review Board. Four patients undergoing prostate biopsy who were negative for cancer were used as case controls to determine the iatrogenic effect of the instrumentation on the normal prostate on the PSA mRNA levels. Peripheral blood samples from 4 normal men and 1 woman were used as negative controls. Patients were followed up by
Sensitivity of PSA RNA detection by 32P-RT-PCR
A semiquantitative 32P-RT-PCR was used to analyze the sensitivity of PSA mRNA detection in total PSA-negative normal female RNA contaminated with the RNA from PSA-positive LNCaP control cells. By this assay, the presence of PSA mRNA expression was detected in as low as 10−7 μg LNCaP RNA in 1 μg of normal female RNA (Fig. 1A). This contamination simulates one tumor cell in 105 mononuclear cells. The PSA/G6PDH densitometry values were directly proportional to the level of contamination by LNCaP
Comment
Radical prostatectomy, brachytherapy, and external beam radiotherapy are currently used for the treatment of localized disease; however, the wide spectrum of biologic behavior exhibited by prostatic neoplasms poses a difficult problem in predicting the clinical course for an individual patient.15 Traditional prognostic markers, such as grade, clinical stage, and pretreatment serum PSA, are used extensively and are of some prognostic value for individual men.16, 17 The pattern of post-treatment
Conclusions
Our results suggest for the first time in published reports that a substantial number of patients undergoing brachytherapy have iatrogenic dissemination of prostate cancer cells in the peripheral blood caused by the insertion of needles in the prostate gland. Also, the detection of PSA mRNA in the peripheral circulation appears to have a significant correlation with biochemical failure after interstitial brachytherapy and needs additional study in a larger patient population.
References (27)
- et al.
The effect of local control on metastatic dissemination in carcinoma of the prostatelong-term results in patients treated with 125I implantation
Int J Radiat Oncol Biol Phys
(1991) Radioisotopic implantation for carcinoma of the prostatedoes it work better than it used to?
Semin Radiat Oncol
(1998)- et al.
Transperineal 125iodine implantation for treatment of clinically localized prostate cancer5-year tumor control and morbidity
Int J Radiat Oncol Biol Phys
(1999) - et al.
Perineal prostatic cancer seeding following radioactive seed brachytherapy
J Urol
(2001) - et al.
Local tract metastasis of prostatic adenocarcinoma 8 years after (125)iodine brachytherapy
J Urol
(2001) - et al.
The correlation between the ASTRO Consensus Panel definition of biochemical failure and clinical outcome for patients with prostate cancer treated with external beam irradiation. American Society of Therapeutic Radiology and Oncology
Int J Radiat Oncol Biol Phys
(1998) - et al.
Scrutiny of the ASTRO consensus definition of biochemical failure in irradiated prostate cancer patients demonstrates its usefulness and robustness. American Society for Therapeutic Radiology and Oncology
Int J Radiat Oncol Biol Phys
(2000) - et al.
Detection of circulating prostate specific antigen expressing prostatic cells in the bone marrow of radical prostatectomy patients by sensitive reverse transcriptase polymerase chain reaction
J Urol
(1999) - et al.
The clinical usefulness of prostate specific antigenupdate 1994
J Urol
(1994) - et al.
Quantitative polymerase chain reaction does not improve preoperative prostate cancer staginga clinicopathological molecular analysis of 121 patients
J Urol
(1996)
Molecular staging of prostate cancer with the use of an enhanced reverse transcriptase-PCR assay
Urology
Preoperative reverse transcriptase polymerase chain reaction for prostate specific antigen predicts treatment failure following radical prostatectomy
J Urol
Prostate cancer and radiation therapy—the message conveyed by serum prostate-specific antigen
Int J Radiat Oncol Biol Phys
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Recovering circulating extracellular or cell-free RNA from bodily fluids
2011, Cancer EpidemiologyCitation Excerpt :The amount of circulating nucleic acids however cannot be justified by the number of cancer cells existing in serum [9], leading to the hypothesis that tumor specific circulating or cell-free nucleic acids come from the necrosis [19,20] or apoptosis [24,25] of cancer cells or they are even actively released by them in microvesicles [26,27,29]. Circulating RNA has been isolated from the plasma or serum of patients suffering from various types of malignancies such as breast cancer [49–56], lung cancer [57–63], prostate cancer [64–78], thyroid cancer [79–98], hepatocellular carcinoma [5,72,75,99–112], melanoma [113–116], gastric cancer [117], renal cell cancer [118], oesophageal cancer [119,120], rectal carcinoma [121], gynecologic malignancies [122], pancreatic cancer [61], colon cancer [61], bladder cancer [16] and solid tumors [123], and has been used as a biological marker either for the early detection and diagnosis of the disease [64,100], or as a marker of recurrence patterns [81,121], survival predictor [51], follow up and surveillance marker [79,82]. In particular, in breast cancer, measurement of serum metastasin mRNA has been proposed as a screening tool, predicting poor survival and distant metastases [51], whereas measurement of circulating 5T4 mRNA as having the potential to identify patients who could benefit from a 5T4-targeted therapy [53].
Circulating tumor cells in patients with solid malignancy treated by high-intensity focused ultrasound
2004, Ultrasound in Medicine and BiologyThe sequence of vessel interruption during lobectomy for non-small cell lung cancer: Is it indeed important?
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2019, Expert Review of Molecular DiagnosticsMolecular mechanisms of metastasis in prostate cancer
2009, Asian Journal of Andrology
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Ayisha Siddiqua was the recipient of the student travel award for this study from the Radiation Research Society.