Elsevier

Human Pathology

Volume 30, Issue 7, July 1999, Pages 849-855
Human Pathology

Original contribution
Histological and immunophenotypic profile of nasal NK/T cell lymphomas from Peru: High prevalence of p53 overexpression,☆☆

https://doi.org/10.1016/S0046-8177(99)90147-8Get rights and content

Abstract

Nasal NK/T-cell lymphoma is a unique form of lymphoma highly associated with Epstein-Barr virus (EBV). These lymphomas are rare in Western populations and much more prevalent in some Asian and Latin American countries. Although there are several sizable studies from Asian countries, the same is not true from South America. The aim of this study was to analyze a series of 32 cases of nasal T-cell lymphoma from Peru and to further extend the characterization of this disease. Immunohistochemistry was performed on paraffin sections using the following antibodies: CD20 (L26), CD45RO, CD3, Ki67, CD57, CD56, TIA-1, bcl-2, and p53. The presence of EBV was investigated with immunohistochemical analysis for latent membrane protein (LMP)-1 and in situ hybridization using an antisense riboprobe to EBER 1. The 32 patients included 18 men and 14 women (M:F ratio, 1.2:1), with a median age of 43 years (11 to 72). Three categories were identified: (1) Nasal NK/T cell lymphomas (28 cases): The morphology ranged from small or medium-sized cells to large transformed cells. Necrosis was present in 86% of the cases, and angioinvasion was seen in 36% of the cases. All cases were positive for CD45RO, CD3, and for TIA-1. CD56 was positive in 21 of 27 cases (78%), and CD57 was negative in all cases. EBER 1 positivity was identified in most of the tumor cells in 27 of 28 cases (96%), including the six cases in which CD56 was negative. Overexpression of p53 was detected in 24 cases (86%). (2) Blastic NK cell lymphoma (1 case): The neoplastic cells resembled those of lymphoblastic lymphoma. CD56 and CD45RO were positive; TIA-1, TdT, and EBER-1 were negative. (3) Peripheral T-cell lymphoma (PTCL) unspecified (3 cases): CD56, TIA-1, and EBER-1 were negative. Nasal lymphomas from Peru with a T cell phenotype are predominantly EBV-associated NK/T cell lymphomas, similar to those described in Asian countries. The expression of CD56, TIA-1, and EBER-1, in combination, are very useful markers for the diagnosis of nasal NK/T cell lymphoma in paraffin-embedded tissue. The differential diagnosis of T-cell lymphomas in the nasal region should include rare cases of PTCL unspecified and the blastic variant of NK cell lymphoma. P53 is overexpressed in 86% of the cases. The significance of this finding with regard to clinical behavior and prognosis remains to be determined.

References (40)

  • C-S Ng et al.

    CD56+ putative natural killer cell lymphomas: Production of cytolytic effectors and related proteins mediating tumor cell apoptosis?

    Hum Pathol

    (1997)
  • ML Gulley et al.

    Epstein-Barr virus infection is associated with p53 accumulation in nasopharyngeal carcinoma

    Hum Pathol

    (1998)
  • DA Arber et al.

    Nasal lymphomas in Peru: High incidence of T-cell immunophenotype and Epstein-Barr virus infection

    Am J Surg Pathol

    (1993)
  • O Misad et al.

    An overview of lymphoreticular malignancies in Peru

    Prog Cancer Res Ther

    (1984)
  • HF Frierson et al.

    Non-Hodgkin's lymphomas of the sinonasal region: Histologic subtypes and their clinicopathologic features

    Am J Clin Pathol

    (1984)
  • FCS Ho et al.

    Clinicopathological features of malignant lymphomas in 294 Hong Kong Chinese patients: Retrospective study covering an eight year period

    Int J Cancer

    (1984)
  • K Aozasa et al.

    Nation-wide study of lethal mid-line granuloma in Japan: Frequencies of Wegener's granulomatosis, polymorphic reticulosis, malignant lymphoma and other related conditions

    Int J Cancer

    (1989)
  • JKC Chan et al.

    Detection of Epstein-Barr viral RNA in malignant lymphomas of the upper aerodigestive tract

    Am J Surg Pathol

    (1994)
  • ES Jaffe et al.
  • WYW Tsang et al.

    Utility of a paraffin section-reactive CD56 antibody (123C3) for characterization and diagnosis of lymphomas

    Am J Surg Pathol

    (1996)
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    Supported in part by the Austrian Science Fund Charlotte-Bühler Habilitationstipendium (LQ-M).

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