Rigid macroporous copolymers as stationary phases in high-performance liquid chromatography

doi:10.1016/S0021-9673(01)83986-8

Journal of Chromatography A

Volume 544, 17 May 1991, Pages 201-217

https://doi.org/10.1016/S0021-9673(01)83986-8 Get rights and content

Abstract

Rigid macroporous copolymers of styrene and divinylbenzene have been developed which have the high physical/mechanical stability necessary to operate under high-performance liquid chromatographic conditions of pressure and flow-rate. The matrices can be produced in a range of porosities, 100, 300, 1000 and 4000 Å each with the controlled uniform pore geometry and pore size distribution essential for high efficiency separations. The small pore—high surface area macroporous poly(styrene—divinylbenzene) copolymer, PLRP-S 100 Å, can be used in the unmodified form for reversed-phase separations of small molecules or by increasing the pore size, PLRP-S 300 Å or greater, for the analysis of biological macromolecules.

The surface characteristics of the hydrophobic poly(styrene-divinylbenzene) matrix can be altered by derivatization or coating to produce ion exchange functionalities or a hydrophilic surface for gel filtration chromatography. The hydrophilic surface can then be further derivatized to be utilised as a base matrix for affinity chromatography.

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ReviewRigid macroporous copolymers as stationary phases in high-performance liquid chromatography

Abstract

J. Biol. Chem.

J. Chromatogr.

Polymer

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

Review
Rigid macroporous copolymers as stationary phases in high-performance liquid chromatography