New methods of measuring the rate of aqueous flow in man with fluorescein

doi:10.1016/S0014-4835(66)80009-X

Experimental Eye Research

Volume 5, Issue 3, July 1966, Pages 208-220

https://doi.org/10.1016/S0014-4835(66)80009-X Get rights and content

Two methods of determining the rate of loss of fluorescein from the aqueous humour in man are described. The dye is introduced into the cornea by means of iontophoresis and estimated in the eye with the slit-lamp fluorophotometer. In the first method the time course of the fluorescein concentration in the anterior chamber is observed, from which its turnover rate in the chamber is derived. In the second method measurements of the ratio of the total fluorescence of the eye to that of the aqueous humour lead to a direct evaluation of the aqueous flow rate. The errors and limitations of the methods are discussed and it is concluded that the second method, though generally more reliable, is inapplicable to blue-eyed subjects. In other cases there is good accordance between the results obtained by the two methods.

In a small group of young normal volunteers the mean turnover rate found by the first method was 0·015 min and the mean rate of flow by the second method 2·5 μl/min.

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New methods of measuring the rate of aqueous flow in man with fluorescein

Exptl Eye Res.

Am. J. Ophthalmol.

Exptl Eye Res.

Brit. J. Ophthalmol.