Inhibition and labeling of the rat renal Na+/H+-exchanger by an antagonist of muscarinic acetylcholine receptors

doi:10.1016/S0006-291X(88)80962-8

Biochemical and Biophysical Research Communications

Volume 157, Issue 3, 30 December 1988, Pages 921-929

https://doi.org/10.1016/S0006-291X(88)80962-8 Get rights and content

A covalently binding label for muscarinic acetylcholine receptors, propylbenzilylcholine mustard (PrBCM), irreversibly inhibits the Na⁺/H⁺ exchanger in rat renal brush-border membrane vesicles. Substrates of the antiporter, Na⁺ and Li⁺, as well as inhibitors, amiloride, 5-(N-ethyl-N-isopropyl)amiloride (EIPA) and propranolol, protect the antiporter from inactivation by PrBCM. With [³H]PrBCM a band with an app. M_r of 65 kDa is predominantly labeled. Amiloride protects this band from labeling with [³H]PrBCM and [¹⁴C]-N,N′-dicyclohexylcarbodiimide (DCCD) proving its identity with the renal Na⁺/H⁺ exchanger. Our data reveal a specific interaction of PrBCM with the Na⁺/H⁺ exchanger and suggest structural relations between antiporter and receptors.

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Inhibition and labeling of the rat renal Na+/H+-exchanger by an antagonist of muscarinic acetylcholine receptors

J. Biol. Chem.

FEBS Letters

Arch. Biochem. Biophys.

J. Biol. Chem.

Biochem. Pharmacol.

J. Biol. Chem.

Kidney Int.

Biochem. Pharmacol.

Biochim. Biophys. Acta

Anal. Biochem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Biochim. Biophys. Acta

Inhibition and labeling of the rat renal Na⁺/H⁺-exchanger by an antagonist of muscarinic acetylcholine receptors