Biochemical and Biophysical Research Communications
Retina-specific expression from the IRBP promoter in transgenic mice is conferred by 212 bp of the 5′-flanking region*
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Optogenetic targeting of AII amacrine cells restores retinal computations performed by the inner retina
2023, Molecular Therapy Methods and Clinical DevelopmentTriple Vectors Expand AAV Transfer Capacity in the Retina
2018, Molecular TherapyCitation Excerpt :In order to test transduction efficiency mediated by triple AAV vectors, we generated a reporter protein by fusing the CDS of EGFP to that of Discosoma red fluorescent protein (DsRed) (herein referred to as ED) separated by a 13 amino acid spacer. A triple flag tag (3xflag) was added at the 3′ end of ED CDS (Figure 1A), which was placed under the control of (1) the ubiquitous cytomegalovirus (CMV) promoter, (2) the PR-specific human interphotoreceptor retinoid-binding protein (IRBP) promoter,41,42 or (3) the RPE-specific vitelliform macular dystrophy 2 (VMD2) promoter.43 The ED cassettes were either packed in a single AAV vector or split in three parts, each packed in a different AAV vector (Figure 1B and Materials and Methods) from here called ED-AAV 1, ED-AAV 2, ED-AAV 3.
Nanoparticles for retinal gene therapy
2010, Progress in Retinal and Eye ResearchCitation Excerpt :The nanoparticles used for these studies were compacted with acetate as the lysine counterion forming rods, and they contained an expression cassette which had 1 of 3 promoters preceding the normal murine RDS cDNA (termed NMP) (Cai et al., 2009a,b). The first promoter derived from the interphotoreceptor retinoid binding protein (IRBP) gene is known to drive gene expression in both rods and cones (Liou et al., 1991; Yokoyama et al., 1992). The second, the mouse opsin promoter (MOP), has been shown to drive very high levels of gene expression in rods with some minimal basal activity in cones, and was chosen because the primary early defect in rds+/− mice occurs in rods (Flannery et al., 1997; Quiambao et al., 1997).
CHX10 targets a subset of photoreceptor genes
2006, Journal of Biological ChemistryCitation Excerpt :DNA recovered from ChIP samples was subjected to real time PCR. The primers were designed for regions upstream of Rod arrestin, Rhodopsin, Red/green cone opsin, and Interphotoreceptor retinoid-binding protein (Irbp) (Fig. 3B), all of which are known to contain homeodomain binding sites (35–38). For negative controls, we amplified the β-globin promoter and the 3′ end of all the retinal target genes we analyzed.
Endogenous CRX expression and IRBP promoter activity in retinoblastoma cells
2001, Brain ResearchCitation Excerpt :IRBP protein is detected only in photosensitive tissues [23] and its mRNA is detected uniquely in photoreceptor cells and pinealocytes [20,21]. The 5′ flanking sequence of the IRBP gene directs promoter activity only in the retina and pineal [3,16]. A short fragment, −123 to +18 relative to transcription start, of the IRBP gene is sufficient to direct retina-specific expression in transgenic mice [5,10].
A PDE6A promoter fragment directs transcription predominantly in the photoreceptor
2001, Biochemical and Biophysical Research Communications
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This study was supported in part by grants from the National Eye Institute (EY03829 to G. I. L. and EY06762 to P. A. O.), Howard Hughes Medical Institute (P. A. O.), and by an Unrestricted Departmental Award from Research to Prevent Blindness, Inc. to the Department of Ophthalmology, Medical College of Georgia.