High performance liquid chromatography (HPLC), also known as high pressure liquid chromatography, is one of the most popular, modern, powerful, and versatile chromatographic separation techniques that have been routinely used to separate the components in a mixture (e.g., an herbal extract or product), to identify each component (or at least as many components as possible), to quantify separated components, and to obtain the chemical profile or fingerprint of a crude mixture. This chapter focuses on the applications of HPLC techniques in the analysis of various herbal products, and it will present several specific examples of protocols of such analyses. A brief overview of available HPLC techniques and methods will also be presented.
