A stereological survey of lysosomal structure alterations in Littorina littorea exposed to 1-naphthol
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2020, Science of the Total EnvironmentEffects of dietary Pb and Cd and their combination on lysosomal and tissue-level biomarkers and histopathology in digestive gland of the land snail, Cantareus apertus (Born, 1778)
2018, Ecotoxicology and Environmental SafetyCitation Excerpt :As a general rule, metal exposure provokes a greater catalytic activity in the digestive cells of molluscs, which might account for changes in lysosomes form and function (Marigomez et al., 1986; Moore and Viarengo, 1990). Thus, lysosomal enlargement in digestive cells is widely recognised as a general indication of environmental stress induced by pollutant (Viarengo et al., 1984; Cajaraville et al., 1989; Guerlet et al., 2006; Marigómez et al., 2005). Lysosomal enlargement (high VvLYS and low S/VLYS) and reduction in numbers (low NvLYS) were reported, associated to intralysosomal accumulation of metals (high VvBSD), in slugs from an abandoned mine in comparison with those form the reference site (Zaldibar et al., 2008).
Differences in copper bioaccumulation and biological responses in three Mytilus species
2015, Aquatic ToxicologyCitation Excerpt :The mean LP value was then derived for each section, corresponding to an individual digestive gland. The histochemical reaction for β-Gus was demonstrated as in Moore (1976) with the modifications described by Cajaraville et al. (1989). Frozen tissue sections (8 μm) from 10 mussels were put at 4 °C for 30 min and then brought to room temperature before staining.
Combined effects of thermal stress and Cd on lysosomal biomarkers and transcription of genes encoding lysosomal enzymes and HSP70 in mussels, Mytilus galloprovincialis
2014, Aquatic ToxicologyCitation Excerpt :Tissue sections (8 μm) were obtained in a Leica CM3050S cryotome with a chamber temperature of −24 °C and stored at −40 °C until required for staining. The histochemical reaction for β-glucuronidase was demonstrated as in Moore (1976) with the modifications described by Cajaraville et al. (1989). Slides were placed at 4 °C for 30 min and then at room temperature for 5 min prior to staining.