Simultaneous isolation of Rutin and Esculin from plant material and drugs using solid-phase extraction

doi:10.1016/0731-7085(93)80199-B

Journal of Pharmaceutical and Biomedical Analysis

Volume 11, Issue 3, March 1993, Pages 211-215

https://doi.org/10.1016/0731-7085(93)80199-B Get rights and content

Abstract

Simultaneous isolation of Rutin and Esculin from pharmaceutical materials (plant — Flos hippocastani and drugs — Venescin®, Venacorn®) using solid-phase extraction (SPE) have been made. For this investigation the Bakerbond SPE columns with different unpolar and polar chemically bonded phases were used. On the basis of isolation investigation the influence of SPE packing materials on the selectivity change and recovery of both extracted substances were studied.

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A landscape analysis of the potential role of polyphenols for the treatment of Polycystic Ovarian Syndrome (PCOS)
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Polycystic Ovarian Syndrome (PCOS) is an under-recognized, under-diagnosed and under-studied disorder; its prevalence varies between 5–26%, 6–10% of reproductive age (NIH) criteria, and 15% (Rotterdam's criteria). Various allopathic drugs (metformin and clomiphene) that are used for the treatment of PCOS have various limitations and are unable to cure PCOS effectively. Therefore, for this reason, search for new therapeutic approaches that are capable of filling these limitations is necessary.
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Sequential high-pressure extraction to obtain capsinoids and phenolic compounds from biquinho pepper (Capsicum chinense)
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Citation Excerpt :
It is an attractive phytochemical due to its pharmacological activities, including antioxidant, anticarcinogenic, neuroprotective and cardioprotective [46,47]. Therefore, rutin is considered as an important flavonoid for the pharmaceutical industry [48]. The relative peak area percentage of the phenolics identified in the base peak chromatogram for all the evaluated PLE conditions is presented in Table S1 of the supplementary material.
This work proposes a sequential high-pressure extraction to recover bioactives from biquinho peppers. The nonpolar fraction was extracted with supercritical carbon dioxide (sc−CO₂). Then, the sc−CO₂ extracted biquinho pepper (EBP) was submitted to pressurized liquid extraction (PLE) using ethanol and water mixtures (50%, 75%, 100% ethanol) at different temperatures (45, 55, 65 °C) to recover compounds of higher polarity. The sc−CO₂ extraction yield was 4.75%, with capsiate concentration of 8.67 mg/g oleoresin. In PLE, the solvent composition influenced the extraction yield and quality. Sixteen phenolics were identified in PLE extracts by UHPLC-MS/MS. Rutin isomer and vicenin-2 were quantified. The highest concentration of rutin isomer (441 μg/g extract) was obtained with pure ethanol whereas, for vicenin-2, the best solvent was 50% ethanol (299 μg/g extract). Considering the extraction of total phenolics, 75% ethanol was the most effective solvent. sc−CO₂ extraction followed by PLE is an interesting alternative to obtain bioactives from peppers.
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Flavonoids play a vital role in the biotechnology industry due to many health benefits. The health benefits have been conferred due to unique properties of flavonoids bio-actives. Such unique properties play a vital role in the free radical scavenging, anti-tumour, anti-atherosclerotic, and anti-diabetic etc. Flavonoids are the diverse bioactive compounds that can be classified into various classes such as flavone, flavonol, flavanol, flavanone, isoflavone, anthocyanidin, and chalcones. Flavonoids can be extracted from a diverse range of sources ranging from microorganisms to higher plants. However, extraction of flavonoids from the native source is a tedious process. Hence, various strategies for the flavonoid extraction has been employed. This chapter discusses the physical and chemical methods employed for flavonoid extraction. Various applications of the flavonoids in the biotechnology sector has been elaborately discussed.
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This paper focuses on the potential corrosion protection of steel in presence of three polyphenolic model molecules (rutin, esculin, esculetol) in a weak acidic medium (pH = 4). From an electrochemical point of view, the cathodic inhibition was attributed to the oxygen scavenger role of the considered antioxidant compounds. SEM observations, Raman spectroscopy and Synchrotron X-ray absorption spectroscopy revealed furthermore the formation of a surface film by precipitation of iron polyphenolate conversion products, in which iron III is likely coordinated with free-catechol groups. Glycosylated catechol groups are rather adsorbed on a FeOOH layer.
Rutin attenuates H<inf>2</inf>O<inf>2</inf>-induced oxidation damage and apoptosis in Leydig cells by activating PI3K/Akt signal pathways
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Oxidative stress is a primary factor in the pathology of male infertility. The strong antioxidative capacity of rutin has been proven by numerous studies, but a protective role in the context of male reproduction remains to be elucidated. To explore the biological role of rutin in protecting male reproductive function and the potential underlying mechanism, H₂O₂-induced Leydig cells were used as a cell model of oxidation damage. Our findings showed that rutin at concentrations of 10, 20, and 40 μmol/L remarkably increased cell survival rate of H₂O₂-induced Leydig cells to 70.1%, 86.8%, and 80.3% respectively. Next, rutin with concentrations of 10, 20, and 40 μmol/L decreased reactive oxygen species (ROS) and malondialdehyde (MDA) levels but increased the levels of glutathione (GSH) and testosterone in H₂O₂-induced Leydig cells. The activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were remarkably increased by rutin treatment with concentrations of 20 and 40 μmol/L, but glutathione peroxidase (GSH-Px) activity was notably decreased. Moreover, rutin with concentrations of 10, 20, and 40 μmol/L increased Bcl-2 protein levels but decreased protein levels of Bax and caspase-3. Furthermore, 20 μmol/L rutin significantly abrogated the decrease in levels of phosphoinositide 3-kinase (PI3K) and phosphorylated serine/threonine kinase (p-AKT) induced by H₂O₂. Pretreatment with LY294002, a PI3K inhibitor, antagonized protective action of 20 μmol/L rutin against H₂O₂-induced cell activities, intracellular oxidant, testosterone, antioxidant enzyme activities, and the apoptosis related protein expression. Taken together, these results suggest that rutin attenuates H₂O₂-induced oxidation damage and apoptosis in Leydig cells by activating PI3K/Akt signal pathways, providing a promising strategy to decrease oxidative stress associated with male infertility.

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^☆: Presented at the “Fourth International Symposium on Drug Analysis”, May 1992, Liège, Belgium.

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Simultaneous isolation of Rutin and Esculin from plant material and drugs using solid-phase extraction☆

Abstract

J. Pharm. Biomed. Anal.

J. Chromatogr.

J. Chromatogr.

Int. Lab.