An enzyme-linked immunosorbent assay for the detection of vesicular stomatitis virus antigen

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Abstract

An indirect, sandwich enzyme-linked immunosorbent assay (ELISA) has been developed for the laboratory diagnosis of vesicular stomatitis (VS). The assay which usesrabbit and guinea-pig antisera to the purified glycoprotein antigens of Serotypes New Jersey (VSV-NJ) and Indiana (VSV-IND-1), has both high sensitivity and specificity, but has lower reactivity for the two other Indiana subtypes VSV-IND-2 and VSV-IND-3.

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Cited by (12)

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    2013, Journal of Virological Methods
    Citation Excerpt :

    VS can be differentiated from other vesicular diseases with a rapid laboratory diagnostic method. An indirect, sandwich enzyme-linked immunosorbent assay (ELISA) has been developed for the laboratory diagnosis of vesicular stomatitis (Ferris and Donaldson, 1988). However, the application of conventional laboratory technologies, such as RT-PCR and viral isolation, are limited by the necessity of skilled technicians or expensive equipment.

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