Elsevier

Gene

Volume 158, Issue 2, 9 June 1995, Pages 247-251
Gene

Short communication
A classical enhancer element responsive to both lipopolysaccharide and interferon-γ augments induction of the iNOS gene in mouse macrophages

https://doi.org/10.1016/0378-1119(94)00892-VGet rights and content

Abstract

The ability of macrophages to kill some kinds of tumor cells is dependent upon the production of the free radical nitric oxide (NO) by the inducible enzyme NO synthase (iNOS; EC 1.14.13.39). Expression of the iNOS gene is induced by lipopolysaccharide (LPS) and augmented by interferon-γ (IFN-γ). Two regions of the iNOS promoter are known to regulate induction, a promoter proximal region I (RI) and a more distal region II (RII). Reconfiguration of RI within the iNOS regulatory region revealed its dependence upon native position and orientation for maximal activity, suggesting that it is a core promoter module, and further implicated the putative octamer element as a contributor to promoter activity. RII, however, functioned in a relatively orientation- and position-independent manner. Therefore, it had the characteristics of a classical enhancer element.

References (19)

There are more references available in the full text version of this article.

Cited by (54)

  • Regulation of extra-renal synthesis of 1,25(OH)<inf>2</inf>D

    2023, Feldman and Pike's Vitamin D: Volume One: Biochemistry, Physiology and Diagnostics
  • Extrarenal 1α-hydroxylase

    2011, Vitamin D: Two-Volume Set
  • Characterization, anti-oxidative and anti-inflammatory effects of Costa Rican noni juice (Morinda citrifolia L.)

    2011, Journal of Ethnopharmacology
    Citation Excerpt :

    NO is a signalling molecule that plays a key role in the pathogenesis of inflammation, and is considered as a pro-inflammatory mediator due to overexpression in abnormal situations. Transcription of iNOS, and thus NO production, is increased in activated macrophages (Alley et al., 2005). We first measured noni juice potential cytotoxic activity on J774 cells by MTS assay.

View all citing articles on Scopus
View full text