Short communicationsNew pUC-derived cloning vectors with different selectable markers and DNA replication origins
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Cited by (462)
Efficient multiplex CRISPR/Cpf1 (Cas12a) genome editing system in Aspergillus aculeatus TBRC 277
2022, Journal of BiotechnologyCitation Excerpt :The plasmids, pCRISPR01 and pCRISPR01-FnCpf1, and pOK12-crRNA-pyrG3 (Abdulrachman et al., 2021) were used to construct CRISPR/Cpf1 plasmids and crRNA cassettes, respectively. Plasmids pOK12 (Vieira and Messing, 1991) and pJET1.2/Blunt (Thermo Fisher, USA) served as cloning vectors. The Cpf1 gene sequence from Acidaminococcus sp.
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Present address: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305 (U.S.A.) Tel. (415)723-1221; Fax (415)725-6757.
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