Brief noteSequence of the precursor of intestinal lactase-phlorizin hydrolase from fetal rat☆
References (5)
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Lactase expression is controlled differently in the jejunum and ileum during development in rats
Gastroenterology
(1991) - et al.
Different expression of the mRNA detected by the cDNA for fetal rat lactase in the jejunum and colon
Gastroenterol. Clin. Biol.
(1990)
Cited by (29)
Regulation of intestine-specific spatiotemporal expression by the rat lactase promoter
2002, Journal of Biological ChemistryCitation Excerpt :For RT-PCR, cDNA was initially synthesized from 1.0 μg of total RNA using avian myeloblastosis virus-reverse transcriptase and the Advantage RT-for-PCR kit (CLONTECH, Palo Alto, CA), according to the protocol of the supplier, and brought to a final volume of 100 μl. PCRs were then carried out with synthetic oligonucleotide primers corresponding to different coding sequences of the firefly luciferase (GenBankTM accession number U47295), lactase (31) (GenBankTM accession number X56747), and glyceraldehyde-3-phosphate dehydrogenase (33) (internal standard, GenBankTM accession number M32599) genes:luc-F, AGATACGCCCTGGTTCCTGG-3′, and luc-R, 5′-ACGAACACCACGGTAGGCTG-3′ (290-bp product); lac-F, 5′-TACCACAAAACCTATATCAACGAGGCT-3′, and lac-R, 5′-CCCGGCCAGGGGCATGCCATTGTTGGCAATGACCTC-3′ (234-bp product);g3pdh-F, 5′-TGAAGGTCGGTGTGAACGGATTTGGC-3′, and g3pdh-R, 5′-CATGTAGGCCATGAGGTCCACCAC-3′ (983-bp product). To prevent nonspecific amplification, hot start PCRs (50 μl) were carried out with 5 μl of the reverse-transcribed cDNA solution: 0.4 μm primers, 0.20 mm dNTPs, 20 mm Tris-HCl (pH 8.4), 50 mm KCl, 1.5 mm MgCl2, and 2.5 units of TaqPolymerase (Invitrogen) preincubated with 1:28 with TaqStart Antibody (CLONTECH).
Species differences in the sites of cleavage of pro-lactase to lactase supports lack of selective pressure
1999, Biochimica et Biophysica Acta - Protein Structure and Molecular EnzymologyProcessing of human intestinal prolactase to an intermediate form by furin or by a furin-like proprotein convertase
1998, Journal of Biological Chemistry
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On request, the authors will supply detailed experimental evidence for the conclusions reached in this brief note.