Elsevier

Gene

Volume 4, Issue 2, October 1978, Pages 121-136
Gene

Construction and characterization of new cloning vehicles III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules

https://doi.org/10.1016/0378-1119(78)90025-2Get rights and content

Abstract

In vitro recombinant DNA techniques were used to construct two new cloning vehicles, pBR324 and pBR325. These vectors, derived from plasmid pBR322, are relaxed replicating elements. Plasmid pBR324 carries the genes from pBR322 coding for resistance to the antibiotics ampicillin (Apr) and tetracycline (Tcr) and the colicin E1 structural and immunity genes derived from plasmid pMBI. Plasmid pBR325 carries the Apr and Tcr genes from pBR322 and the cloramphenicol resistance gene (Cmr) from phage P1Cm. In these plasmids the unique EcoRI restriction site present in the DNA molecule is located either in the colicin E1 structural gene (pBR324) or in the Cmr gene (pBR325). These vectors were constructed in order to have a single EcoRI site located in the middle of a structural gene which when inactivated would allow, for the easy selection of plasmid recombinant DNA molecules. These plasmids permit the molecular cloning and easy selecion of EcoRI, BamHI, HindIII, PstI, HincII, SalI, (XamI), SmaI, (XmaI)), BglII and DpnII restriction generated DNA molecules.

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