Inhibitory effect of the endothelium on thrombin-induced contraction in rabbit aorta

https://doi.org/10.1016/0306-3623(91)90569-RGet rights and content

Abstract

1. Thrombin caused a tonic contractile response in rabbit aortic strips which showed tachyphylaxis.

2. Thrombin-induced contraction was partially dependent upon extracellular calcium.

3. Contractile response by lower concentrations of thrombin was suppressed by the endothelium. This endothelial effect was blocked by methylene blue, hemoglobin, bromophenacyl bromide or removal of extracellular calcium but not by indomethacin, nordihydroguaiaretic acid or nifedipine.

4. Cyclic GMP levels were not different between the thrombin-stimulated and control strips.

5. Thrombin could not stimulate prostacyclin release from the aortic strips.

6. These results suggest that thrombin possesses a contractile action in rabbit aortic smooth muscle which is attenuated by endothelium-derived relaxing factor (EDRF) spontaneously released from the endothelium during the contraction.

References (31)

  • D. Gebremedhin et al.

    Species specificity of thrombin-induced changes in vascular tone

    Eur. J. Pharmac.

    (1986)
  • H. Moritoki et al.

    Age-related decrease in endothelium-dependent dilator response to histamine in rat mesenteric artery

    Eur. J. Pharmac.

    (1986)
  • T.M. Cocks et al.

    Endothelium-dependent relaxation of coronary arteries by noradrenaline and serotonin

    Nature

    (1983)
  • J.G. DeMey et al.

    Heterogenous behavior of the canine arterial and venous wall

    Circulation Res.

    (1982)
  • J.G. DeMey et al.

    Endothelium-dependent inhibitory effects of acetylcholine, adenosine triphosphate, thrombin and arachidonic acid in the canine femoral artery

    J. Pharmac. exp. Ther.

    (1982)
  • R.F. Furchgott

    Role of endothelium in responses of vascular smooth muscle

    Circulation Res.

    (1983)
  • R.F. Furchgott et al.

    The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine

    Nature

    (1980)
  • C.J. Garland et al.

    Alpha adrenoceptor antagonists selectively reduce thrombin-stimulated contraction in rabbit arteries

    J. Pharmac. exp. Ther.

    (1986)
  • E. Glusa et al.

    The contractile response of vascular smooth muscle to thrombin and its inhibition by thrombin inhibitor

    Folia Haematol.

    (1988)
  • D.G. Harrison et al.

    Alterations of vascular reactivity in atherosclerosis

    Circulation Res.

    (1987)
  • K. Hatake et al.

    Effect of aging on endothelium-dependent vascular relaxation of isolated human basilar artery to thrombin and bradykinin

    Stroke

    (1990)
  • V.M. Haver et al.

    Generation of a vasoactive substance in human plasma during coagulation

    Evidence of thrombin-induced contraction of rabbit aorta and dog coronary artery

    Blood Vessels

    (1983)
  • W.J. Janssens et al.

    Effect of thrombin on isolated canine blood vessels

    Blood Vessels

    (1982)
  • R.L. Jayakody et al.

    Absence of effect of calcium antagonists on endothelium-dependent relaxation in rabbit aorta

    Br. J. Pharmac.

    (1987)
  • P. Kim et al.

    Alterations in endothelium-dependent responsiveness of the canine basilar artery after subarachinoid hemorrhage

    J. Neurosurg.

    (1988)
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