General paper
Characterization of a chymosin-like pepsin from the dogfish Scyliorhinus canicula

https://doi.org/10.1016/0305-0491(87)90250-1Get rights and content

Abstract

  • 1.

    1. Pepsin II extracted from the gastric mucosa of Scyliorhinus canicula has been characterized and compared to calf chymosin.

  • 2.

    2. The kcat and Km of the dogfish enzyme for the synthetic hexapeptide Leu-Ser-Phe(NO2)-Nle-Ala-Leu-OMe have been determined. The kcat/Km ratio is close to that of calf chymosin. Its milk-clotting efficiency is however 21-fold lower than that of calf chymosin.

  • 3.

    3. The proteolytic activity against haemoglobin is optimal at pH 2.5. It clots the milk up to pH 6.8.

  • 4.

    4. The dogfish pepsin II shows relatively better activity at low temperatures than calf chymosin.

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