Comparative Biochemistry and Physiology Part A: Physiology
Sodium uptake and transport (Na+ + K+)ATPase changes following Na+ depletion and low salinity acclimation in the mangrove crab Ucides cordatus (L.)
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Cited by (35)
Osmotic and ionic regulation, and modulation by protein kinases, FXYD2 peptide and ATP of gill (Na<sup>+</sup>, K<sup>+</sup>)-ATPase activity, in the swamp ghost crab Ucides cordatus (Brachyura, Ocypodidae)
2020, Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular BiologyCitation Excerpt :Hemolymph [Na+] ranges from 300 to 390 mmol L−1 at salinities above 34‰S (Santos and Salomão, 1985b). Acclimation of U. cordatus to dilute seawater increases (Na+, K+)-ATPase activity by ≈1.5-fold in the posterior gills and by ≈2-fold in the antennal glands (Harris and Santos, 1993b). However, while osmoregulatory ability seems well characterized (Santos and Salomão, 1985a, 1985b), little is known of the biochemical processes underlying ion transport in U. cordatus gills.
Gill-specific (Na<sup>+</sup>, K<sup>+</sup>)-ATPase activity and α-subunit mRNA expression during low-salinity acclimation of the ornate blue crab Callinectes ornatus (Decapoda, Brachyura)
2015, Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular BiologyCitation Excerpt :The outside-positive transepithelial potential may also drive inward paracellular Na+ flow across this low resistance epithelium (Onken et al., 2003; Freire et al., 2008). Posterior gills exhibit higher (Na+, K+)-ATPase activity than anterior gills (Harris and Santos, 1993; Lopez-Mañanes et al., 2002) owing in part to the greater abundance of α-subunit mRNA in their total RNA extracts (Towle et al., 2001; Weihrauch et al., 2004). Euryhaline crabs exhibit adjustments in gill (Na+, K+)-ATPase activity and α-subunit mRNA expression particularly in response to lowered salinity (Lovett et al., 2006; Masui et al., 2009).
Black yeast-like fungi associated with Lethargic Crab Disease (LCD) in the mangrove-land crab, Ucides cordatus (Ocypodidae)
2012, Veterinary MicrobiologyCitation Excerpt :CBS 119710 (T) were plated on Brain Heart Infusion Agar (BHI) and incubated at 25 °C to obtain active cultures. Conidia and hyphal elements collected from the surface of fresh colonies were resuspended by shaking on a Vortex laboratory shaker in saline solution (2.5%, matching U. cordatus hemolymph physiological salinity) (Harris and Santos, 1993) with 1% Tween®20 (Promega, Madison, WI, USA). Subsequently, the suspension containing 2 × 107 elements per ml was prepared for inoculation.
Viability of the etiologic agent of the Lethargic Crab Disease, Exophiala cancerae, during cooking of the mangrove-land crab: Does this traditional dish represent a risk to humans?
2012, Food ControlCitation Excerpt :This strain was isolated and purified from moribund crab tissues during an LCD’s mortality outbreak in the state of Sergipe, northeast Brazil. The yeast cells and elements of hyphae obtained from the recovered colonies were suspended in saline solution (2.5%, the same physiological salinity of U. cordatus hemolymph–Harris & Santos, 1993), resulting in a stock solution of approximately 2 × 107 fungal elements per mL. Manipulations were performed in a horizontal laminar air-flow workstation (LabCon Co., Purifier Class II).
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