Elsevier

Vaccine

Volume 12, Issue 11, 1994, Pages 993-999
Vaccine

Paper
An early humoral immune response in peripheral blood following parenteral inactivated influenza vaccination

https://doi.org/10.1016/0264-410X(94)90334-4Get rights and content

Abstract

The enzyme-linked immunospot assay was used to examine the humoral immune response in 15 healthy volunteers immunized with either split or subunit inactivated trivalent influenza vaccine containing A/Beijing/353/89 (H3N2), A/Taiwan/1/86 (H1N1) and B/Yamagata/16/88. The rapidity of the individual B-cell and serum antibody response was examined in lymphocyte and serum samples collected at various time intervals after vaccination. A rapid serological response was detected with increases in antibody titre detected in the majority of volunteers by 7–8 days postvaccination. Influenza-specific plasma cells were detected as early as 4 days postvaccination, higher numbers of IgA and IgG antibody-secreting cells (ASC) were observed which peaked at 7–8 days postvaccination. The number of ASCs then declined, with low numbers of cells detected at 11 days postvaccination. Influenza-specific IgA ASCs were predominantly of the IgA1 subclass. This rapid immune response may have a bearing on future vaccination policies of unimmunized ‘at risk groups’ in times of high influenza activity.

References (30)

  • D.C. Powers et al.

    Systemic and local antibody responses in elderly subjects given live or inactivated influenza A virus vaccines

    J. Clin. Microbiol.

    (1989)
  • M.A. Zuckerman et al.

    Serological responses in volunteers to inactivated trivalent subunit influenza vaccine: antibody reactivity with epidemic influenza A and B strains and evidence of a rapid immune response

    J. Med. Virol.

    (1991)
  • M.L. Clements et al.

    Resistance of adults to challenge with influenza A wild-type virus after receiving live or inactivated virus vaccine

    J. Clin. Microbiol.

    (1986)
  • C. Czerkinsky et al.

    Antibody-producing cells in peripheral blood and salivary glands after oral cholera vaccination of humans

    Infect. Immun.

    (1991)
  • A. Kantele et al.

    Specific immunoglobulin-secreting human blood cells after peroral vaccination against Salmonella typhi

    J. Infect. Dis.

    (1986)
  • Cited by (138)

    • Potential bovine colostrum for human and animal therapy

      2021, Current Developments in Biotechnology and Bioengineering: Technologies for Production of Nutraceuticals and Functional Food Products
    • Impact of pre-existing immunity on the induction of functional cross-reactive anti-hemagglutinin stalk antibodies following vaccination with an AS03 adjuvanted pandemic H1N1 vaccine

      2018, Vaccine
      Citation Excerpt :

      Titers <10 were assigned a value of 5 for calculation purposes. Sera were evaluated in duplicate for IgG1, IgG2, IgG3 and IgG4 antibodies [12]. The plates were coated with capture IgG antibody (0.3 µg/ml), a proxy influenza HA1 head (A/California/06/2009(H1N1)) hexahistidine-tagged (1 μg/ml) (eEnzyme, IA-01SW-005P) or 1 μg/ml influenza cH6/1, a chimeric HA (cHA) that combines the H1 stalk domain with the globular head domain derived from H6 influenza A virus [13].

    View all citing articles on Scopus
    View full text