PaperAn early humoral immune response in peripheral blood following parenteral inactivated influenza vaccination
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2018, VaccineCitation Excerpt :Titers <10 were assigned a value of 5 for calculation purposes. Sera were evaluated in duplicate for IgG1, IgG2, IgG3 and IgG4 antibodies [12]. The plates were coated with capture IgG antibody (0.3 µg/ml), a proxy influenza HA1 head (A/California/06/2009(H1N1)) hexahistidine-tagged (1 μg/ml) (eEnzyme, IA-01SW-005P) or 1 μg/ml influenza cH6/1, a chimeric HA (cHA) that combines the H1 stalk domain with the globular head domain derived from H6 influenza A virus [13].
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