cDNA sequence for bovine ribosomal protein L3 carrying a bipartite nuclear targeting motif, identified also in many other ribosomal proteins

doi:10.1016/0167-4781(94)90232-1

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression

Volume 1219, Issue 3, 22 November 1994, Pages 706-710

https://doi.org/10.1016/0167-4781(94)90232-1 Get rights and content

Abstract

A full-length cDNA encoding bovine ribosomal protein L3 was isolated and sequenced. The deduced protein sequence comprises 403 amino acids and shows a high level of identity with the other known mammalian L3 proteins. Southern blot analysis of bovine genomic DNA suggests that the bovine genome contains at least 4 copies of the L3 gene. A single hybridisation band of about 1.3 kb is detectable by Northern blot analysis. Within the amino acid sequence, two potential nuclear targeting sequences were detected: one at the N-terminal end and the other, consisting in a bipartite motif (amino acids 341 to 358), present and not previously noticed also in the other known mammalian L3 proteins. A search on all the available mammalian ribosomal proteins revealed the presence of this bipartite motif in many of these proteins.

References (17)

Y. Kuwano et al.
Biochem. Biophys. Res. Commun.
(1992)
P. Chomczynski et al.
Anal. Biochem.
(1987)
Y. Kim et al.
Gene
(1990)
R.J. Monk et al.
Cell
(1981)
Y.L. Chan et al.
J. Biol. Chem.
(1988)
J. Robbins et al.
Cell
(1991)
C. Dingwall et al.
Trends Biochem. Sci.
(1991)
J. Garcia-Bustos et al.
Biochim. Biophys. Acta
(1991)

There are more references available in the full text version of this article.

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Citation Excerpt :
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^☆: The nucleotide sequence data reported in this paper have been submitted to the EMBL/GenBank Data Libraries under the accession number Z29555.

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Short sequence-papercDNA sequence for bovine ribosomal protein L3 carrying a bipartite nuclear targeting motif, identified also in many other ribosomal proteins☆

Abstract

Biochem. Biophys. Res. Commun.

Anal. Biochem.

Gene

Cell

J. Biol. Chem.

Cell

Trends Biochem. Sci.

Biochim. Biophys. Acta

Short sequence-paper
cDNA sequence for bovine ribosomal protein L3 carrying a bipartite nuclear targeting motif, identified also in many other ribosomal proteins☆