The detection of foot-and-mouth disease virus in oesophagealpharyngeal samples by a polymerase chain reaction technique
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Development of one-step multiplex RT-PCR method for simultaneous detection and differentiation of foot-and-mouth disease virus serotypes O, A, and Asia 1 circulating in Vietnam
2011, Journal of Virological MethodsCitation Excerpt :Many factors, such as specific primers, PCR-related reagents and conditions, quality of samples, virus strains used, can influence the sensitivity of the RT-PCR (Reid et al., 1998). The detection limits of PCR for FMDV detection have been described in several previous reports and it was shown that the sensitivity of the PCR, depending on different primer pairs used, varied between 0.01 TCID50 to 104 TCID50 (Amaral-Doel et al., 1993; Clavijo et al., 2003; Giridharan et al., 2005; House and Meyer, 1993; Meyer et al., 1991; Rodriguez et al., 1994). In this report, the limits of FMDV detection of one-step multiplex RT-PCR using the new primers varied between 101.5 and 103.5 TCID50/ml, it was most sensitive for serotypes A (A22/Iraq), followed by serotype O (O1/Manisa), and Asia 1 (As1/Shamir/89).
Development and evaluation of a multiplex PCR for differentiation of foot-and-mouth disease virus strains native to India
2005, Journal of Virological MethodsThe pathogenesis and diagnosis of foot-and-mouth disease
2003, Journal of Comparative PathologyAspects of the persistence of foot-and-mouth disease virus in animals - The carrier problem
2002, Microbes and InfectionPicornaviruses
2019, Diseases of SwineFoot-and-mouth disease virus carrier status in Bos grunniens yaks
2013, Virology Journal