A new spectrophotometric cuvette holder for low temperature studies; Its application to the study of carbonmonoxyhemoglobin oxidation rate

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Abstract

A new spectrophotometric cuvette holder to be used to subzero temperature is described. The device is easily adaptable to a commercial spectrophotometer and it was checked down to −40°C. Satisfactory mixing of the reactants contained in the cuvette at low temperatures is attained using a special stirrer and suitable solution volumes. The rate of carbonmonoxyhemoglobin oxidation by K3Fe(CN)6 at different subzero temperatures has been studied using this apparatus; the results are in aggrement with the extrapolated data at room temperature.

References (6)

  • P. Maurel et al.

    Anal. Biochem.

    (1974)
  • V.S. Sharma et al.

    J. Biol. Chem.

    (1976)
  • J.V. Kilmartin et al.

    Biochem. J.

    (1971)
There are more references available in the full text version of this article.

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