Cell

Cell

Volume 71, Issue 6, 11 December 1992, Pages 963-972
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Article
Expression of a membrane protease enhances presentation of endogenous antigens to MHC class I-restricted T lymphocytes

https://doi.org/10.1016/0092-8674(92)90392-PGet rights and content

Abstract

We find that expression of the membrane dipeptidyl carboxypeptidase angiotensin-converting enzyme (ACE) enhances presentation of certain endogenously synthesized peptides to major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes. ACE appears to function only in an intracellular secretory compartment of antigen-presenting cells. ACE-enhanced antigen presentation requires the expression of the putative antigenic peptide transporters, TAP1 and TAP2. These findings demonstrate that a protease can influence the processing of endogenously synthesized antigens and strongly suggest that longer peptides can be transported from the cytosol to a secretory compartment where trimming of antigenic peptides to the lengths preferred by MHC class I molecules can occur if the appropriate protease is present.

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    †

    Present address: Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.

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