Temporal fluctuations in HIV quasispecies in vivo are not reflected by sequential HIV isolations

doi:10.1016/0092-8674(89)90942-2

Cell

Volume 58, Issue 5, 8 September 1989, Pages 901-910

https://doi.org/10.1016/0092-8674(89)90942-2 Get rights and content

Abstract

A genetic study has been made of the HIV tat gene from sequential HIV-1 isolates and the corresponding infected peripheral blood mononuclear cells. DNA was amplified by polymerase chain reaction (PCR) and cloned into a eukaryotic expression vector. Twenty clones were sequenced from each sample. Comparing the sequential HIV isolates, abrupt differences were seen between the major forms of each isolate. These progressive changes were not reflected at all among the in vitro samples. The fluctuation in the quasispecies in vivo may suggest a much more dynamic role for Iatently infected mononuclear cells. High frequencies of functionally defective tat genes were identified. Given such complexity and the evident differences between quasispecies in vivo and in vitro, the task of defining HIV infection in molecular terms will be difficult.

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Cell

ArticleTemporal fluctuations in HIV quasispecies in vivo are not reflected by sequential HIV isolations

Abstract

Cell

Lancet

J. Virol. Meth.

Cell

Meth. Enzymol.

Cell

Virology

Anal. Biochem.

Cell

Cell

Severe immunodeficiency disease induced by a defective murine leukaemia virus

Nature

Defective interfering viruses and infections of animals

Cur. Topics Microbiol. Immunol.

Buffer gradient gels and 35S Iabel as an aid to rapid DNA sequence determination

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Nucl. Acids Res.

Biologic features of HIV-1 that correlate with virulence in the host

Science

Genetic recombination of human immunodeficiency virus

J. Virol.

RNA Genetics

Determination of the rate of base-pair substitution and insertion mutations in retrovirus replication

J. Virol.

Self organization of matter and the evolution of biological macromolecules

Naturwissenschaften

The specificity of the human immunodeficiency virus type 2 transactivator is different from that of human immunodeficiency virus type 1

EMBO J.

Distinct replicative and cytopathic characteristics of human immunodeficiency virus isolates

J. Virol.

Biologically diverse molecular variants within a single HIV-1 isolate

Nature

Tat protein from human immunodeficiency virus forms a metal-linked dimer

Science

Expression of a truncated viral trans-activator selectively impedes lytic infection by its cognate virus

Nature

Functional domains required for tat-induced transcriptional activation of the HIV-1 long terminal repeat

EMBO J.

HIV-1 isolates are rapidly evolving quasispecies: evidence for viral mixtures and preferred nucleotide substitutions

J. AIDS

Article
Temporal fluctuations in HIV quasispecies in vivo are not reflected by sequential HIV isolations

Buffer gradient gels and ³⁵S Iabel as an aid to rapid DNA sequence determination