Fluorogenic and chromogenic glycosides as substrates and ligands of carbohydrases
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Kinetic and molecular dynamics study of inhibition and transglycosylation in Hypocrea jecorina family 3 -glucosidases
2019, Journal of Biological ChemistryCitation Excerpt :Cellobiose was purchased from CU Chemie Uetikon (Lahr, Germany), glucose was from Janssen Pharmaceuticals (New Brunswick, NJ), laminaribiose was from Megazyme (Wicklow, Ireland), gentiobiose and sophorose were from Sigma, and cello-oligosaccharides were from Merck. CNPG and CNPX were synthesized as described previously (33, 34). The purified β-glucosidases HjCel3A and HjCel3B were a gift from DuPont Industrial Biosciences.
Xylans inhibit enzymatic hydrolysis of lignocellulosic materials by cellulases
2012, Bioresource TechnologyCitation Excerpt :Protein was quantified by the Lowry method, using bovine serum albumin (Sigma Chemical Co., USA) as standard (Lowry et al., 1951). Cellobiohydrolase I activity was determined using 4-methylumbelliferyl-β-d-lactoside (MUL) as substrate according to van Tilbeurgh et al. (1982, 1988). Soluble and insoluble oat spelt xylans were prepared by a modified method of Ryan et al. (2003).
Xylo-oligosaccharides are competitive inhibitors of cellobiohydrolase I from Thermoascus aurantiacus
2012, Bioresource TechnologyEnzymatic accessibility of fiber hemp is enhanced by enzymatic or chemical removal of pectin
2012, Bioresource TechnologyThe laccase-catalyzed modification of lignin for enzymatic hydrolysis
2011, Enzyme and Microbial TechnologyCitation Excerpt :The elution was performed with 2 mM NaOH at 1 ml/min with a post-column addition of 300 mM NaOH at 0.3 ml/min. The cellobiohydrolase (CBH) activity was measured using 4-methylumbelliferyl-β-d-lactoside (MUL) as substrate, according to van Tilbeurgh et al. [28]. The endoglucanase (EG) activity was assayed using 1% hydroxyl ethyl cellulose as substrate [29].