RNA polymerase activity and protein synthesis in brome mosaic virus-infected protoplasts

doi:10.1016/0042-6822(79)90002-3

Virology

Volume 99, Issue 2, December 1979, Pages 218-225

https://doi.org/10.1016/0042-6822(79)90002-3 Get rights and content

Abstract

RNA-dependent RNA polymerase activity and protein synthesis in brome mosaic virus (BMV)-infected and uninfected protoplasts were investigated during the course of viral replication. In protoplast homogenates, the membrane-bound RNA polymerase activity resistant to actinomycin D was enhanced by BMV infection up to 30-fold that found in mock-inoculated protoplasts. The activity was first detected 7 to 8 hr postinfection. It reached a maximum at around 30 hr postinfection, then decreased gradually while virus and the viral antigen in the protoplasts, as measured by infectivity and fluorescent antibody staining, respectively, continued to increase. The bound enzyme activity was not enhanced by inoculation with BMV RNA 3 and/or RNA 4. Four proteins with molecular weights of 120, 110, 36, and 19.5 × 103 were observed in BMV-infected protoplasts from three plant species (a systemic host, a local lesion host, and a nonhost of BMV).

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Viral protein synthesis in Barley protoplasts inoculated with native and fractionated brome mosaic virus RNA
1981, Virology
When barley protoplasts were inoculated with brome mosaic virus (BMV) RNAs 1 and 2, there was a pronounced synthesis of the 110,000- and 100,000-dalton virally coded proteins. In contrast, there was no detectable synthesis of any viral proteins following inoculation with RNA 3 alone or RNA 4 alone. When RNAs 1 and 2 were recombined with RNA 3 in the inoculum, the profile of proteins synthesized was identical to that following inoculation with similar quantities of unfractionated BMV RNA; i.e., the 35,000-dalton virally coded protein and coat protein were synthesized in addition to the two high-molecular-weight viral polypeptides. RNAs 1 and 2 were shown not to be selectively bound (in preference to RNAs 3 or 4); hence, these data reveal that one or both of these RNAs encode proteins involved in early events of infection, perhaps replication.
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Some suggestions concerning the mechanism of synthesis as well as the nature of the J proteins were made.

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¹: Present address: Department of Plant Science, University of Alberta, Edmonton, Alberta T6G 2E3, Canada.

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RNA polymerase activity and protein synthesis in brome mosaic virus-infected protoplasts

Abstract

Virology

Advan. Virus Res.

Virology

Virology

J. Mol. Biol.

Infectivity studies of bromegrass mosaic virus RNA

Virology

RNA polymerase from tobacco necrosis virus infected and uninfected tobacco: Purification of the membrane-associated enzyme

Virology

Infection with BMV of mesophyll protoplasts isolated from five plant species

J. Gen. Virol.

Properties of single- and doublestranded rivonucleic acid from barley plants infected with bromegrass mosaic virus

J. Virol.

Properties of single-stranded RNA synthesized by a crude RNA polymerase fraction from barley leaves infected with brome mosaic virus

J. Gen. Virol.

Cleavage of structural proteins during the assembly of the head of bacteriophage T4

Nature (London)