Journal of Molecular Biology
Bacteriophage P1 cre gene and its regulatory region: Evidence for multiple promoters and for regulation by DNA methylation☆
References (44)
- et al.
Gene
(1983) - et al.
J. Biol. Chem.
(1984) - et al.
Cell
(1983) - et al.
Cell
(1981) - et al.
Gene
(1979) - et al.
Virology
(1976) - et al.
Virology
(1977) - et al.
Virology
(1983) - et al.
J. Mol. Biol.
(1985) - et al.
Anal. Biochem.
(1981)
J. Mol. Biol.
Virology
Virology
Virology
J. Mol. Biol.
J. Mol. Biol.
Gene
J. Mol. Biol.
Gene
Virology
Genetics
Cited by (138)
The history of genome editing: advances from the interface of chemistry & biology
2023, Chemical CommunicationsConstruction of chimeric viruses based on pepper mild mottle virus using a modified Cre/loxP system
2022, Journal of Integrative AgricultureCitation Excerpt :The bacteriophage P1 Cre/loxP site-specific recombination system, which is used to integrate DNA specifically at loxP sites, is a versatile tool for modifying DNA in both mammalian systems and plants (Lutz et al. 2006; Cao et al. 2010; Wachsman and Heidstra 2010; Kasai and Harayama 2016). The Cre/loxP system utilizes the 38-kDa recombinase subunits and the 34-bp loxP sites, without the need for other accessory factors (Sternberg et al. 1986). Recombination between two loxP sites in the same orientation results in the excision of the DNA segment, whereas recombination between inverted loxP sites produces an intervening DNA segment.
Simultaneous resistance against the two viruses causing rice tungro disease using RNA interference
2018, Virus ResearchCitation Excerpt :Artificial triggering of RNAi by the expression of intron hairpin RNA (ihpRNA), double-stranded RNA (dsRNA), and by other similar strategies have been used as a means to successfully engineer virus resistance in plants in the past decade and a half (Waterhouse et al., 1998; Pooggin et al., 2003; Tenllado et al., 2003; Lennefors et al., 2006; Tougou et al., 2006; Bonfim et al., 2007; Tyagi et al., 2008; Shimizu et al., 2012; Le et al., 2015; Singh et al., 2015; Wang et al., 2016). For easier regulatory approvals and better consumer acceptance of the product, the site-specific recombination system Cre/LoxP from bacteriophage P1 (Sternberg et al. 1986; Yarmolinsky and Sternberg, 1988) has been widely used for the generation of marker-free transgenic plants, intially in model plants such as Arabidopsis (Zuo et al., 2001; Hoff et al., 2001) and Nicotiana tabacum (Odell et al., 1990; Dale and Ow, 1991) and later, in rice. These were achieved either using sexual-crossing (Hoa et al., 2002; Sengupta et al., 2010) or auto-excision approaches (Sreekala et al., 2005; Bai et al., 2008).
Transgenesis and Future Applications for Cavefish Research
2016, Biology and Evolution of the Mexican CavefishModification of the genome of rhodobacter sphaeroides and construction of synthetic operons
2011, Methods in Enzymology
- ☆
This work was supported by The National Cancer Institute, DHHF, under contract no. NO1-CO-23909 with Litton Bionetics Incorporated.
- †
Present address: E. I. Du Pont de Nemours and Company, Central Research & Development Department, Experimental Station, E328/145, Wilmington, DE 19898, U.S.A.