Expression of cytokine-induced neutrophil chemoattractant in rat cardiac myocytes

doi:10.1016/0022-2828(95)90025-X

Journal of Molecular and Cellular Cardiology

Volume 27, Issue 9, September 1995, Pages 2043-2051

https://doi.org/10.1016/0022-2828(95)90025-X Get rights and content

Abstract

Accumulation and adhesion of leukocytes to cardiac myocytes play important roles in the pathogenesis of inflammation-mediated myocardial injury such as ischaemia/reperfusion and myocarditis. The involvement of leukocyte chemotactic factors has been speculated in these processes. We investigated the expression of cytokine-induced neutrophil chemoattractant (CINC) in rat cardiac myocytes. CINC is a rat equivalent of human interleukin-8. On exposure to interleukin-1α (IL-1α), cultured neonatal rat cardiac myocytes released appreciable levels of CINC both dose-and time-dependently. Tumor necrosis factor-α and lipopolysaccharide also significantly increased CINC accumulation in the culture supernatant. CINC mRNA expression was not observed in unstimulated myocytes, however, the expression was markedly induced by exposure to IL- 1α with a peak elevation at 3 h. Potent chemotactic activity for neutrophils was detected in the supernatant of cultured rat cardiac myocytes by stimulation with IL-1α. This IL-1α-induced chemotactic activity was significantly inhibited by polyclonal anti-CINC antiserum. Addition of dexamethasone, genistein, actinomycin D or cycloheximide significantly suppressed the IL-1α-induced CINC accumulation. Under hypoxia (95%N₂+5%CO₂), CINC accumulation was increased in a time-dependent manner, and reoxygenation after hypoxia further intensified CINC accumulation. This hypoxia reoxygenation-induced CINC expression was significantly inhibited by pretreatment with dexamethasone. In conclusion, inflammatory stimuli induce the expression of CINC in rat cardiac myocytes, which may lead to myocardial injury via accumulation and activation of neutrophils.

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Original articleExpression of cytokine-induced neutrophil chemoattractant in rat cardiac myocytes

Abstract

J Mol Cell Cardiol

J Biol Chem

Am Heart

Am J Cardiol

Cytokine

Transient rise in serum interleukin-8 concentration during acute myocardial infarction

Br Heart

Nafazatrom-induced savage of ischemic myocardium in anesthetized dogs is mediated through inhibition of neutrophil function

Circ Res

Interleukin 8 production by human peritoneal mesothelial cells in response to tumor necrosis factor-α, interleukin-1, and medium conditioned by macrophages cocultured with staphylococcus epidermidis

J Inject Dis

Investigation of possible autocrine functions for rat GRO/CINC (cytokine-induced neutrophil chemoattractant)

J Cell Phsyiol

Leukocytes capillary plugging in myocardial ischemia and reperfusion in the dog

Am J Pathol

Induction of neutrophil infiltration by rat chemotactic cytokine (CINC) and its inhibition by dexamethasone in rats

Inflammation

Level of neutrophil chemotactic factor CINC/gro, a member of the interleukin-8 family, associated with lipopolysaccharide-induced inflammation in rats

Infect Immun

Induction of interleukin-8 expression during cardiopulmonary bypass

Circulation

Regulation of Na,K-ATPase gene expression by thyroid hormone in rat cardiocytes

Circ Res

Hypoxic induction of interleukin-8 gene expression in human endothelial cells

J Clin Invest

Elevation of cytokines during open heart surgery with cardiopulmonary bypass: participation of interleukin 8 and 6 in reperfusion injury

Can J Anaesth

Original article
Expression of cytokine-induced neutrophil chemoattractant in rat cardiac myocytes