Elsevier

Journal of Chromatography A

Volume 645, Issue 2, 20 August 1993, Pages 201-207
Journal of Chromatography A

Laser-based double beam absorption detector for high-performance liquid chromatography

https://doi.org/10.1016/0021-9673(93)83379-7Get rights and content

Abstract

The performance of a new double-beam absorption detector based on an all-electronic noise canceler was tested under HPLC conditions. The test compounds were chosen to be 2′-Cl-4-dimethylaminoazobenzene and 4′-Cl-4-dimethylaminoazobenzene since they have absorption peaks in both the UV and the visible region. The major properties of the detector are discussed. The detector is linear through almost 4 orders of magnitude (10−5-10−9M). The deviation between consecutive measurements is about 3% and the absorption noise level is 2 · 10−6 AU, which is lower by one order of magnitude compared to commercial absorption detectors.

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