Determination of cannabinoid acids by high-performance liquid chromatography of their neutral derivatives formed by thermal decarboxylation: I. Study of the decarboxylation process in open reactors

doi:10.1016/0021-9673(90)85118-F

Journal of Chromatography A

Volume 520, 9 November 1990, Pages 339-347

https://doi.org/10.1016/0021-9673(90)85118-F Get rights and content

Abstract

Decarboxylation of cannabidiolic and tetrahydrocannabinolic acids was studied in open reactors in order to investigate the accuracy and reliability of the decarboxylation sample preparation process applied prior to indirect methods, which has been widely used for the determination of cannabinoid acids. The rate of the decarboxylation reaction was followed by the high-performance liquid chromatographic determination of the neutral cannabinoids formed. The effects of different parameters (temperature, solvents, sorbent phases, salts) on decarboxylation were investigated. Reliable results could only be obtained by the mathematical correction of data obtained from experiments in an open reactor.

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Determination of cannabinoid acids by high-performance liquid chromatography of their neutral derivatives formed by thermal decarboxylation: I. Study of the decarboxylation process in open reactors

Abstract

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

Lloydia