Fasciola hepatica: Increase of glycogen phosphorylase activity due to prostaglandins

doi:10.1016/0014-4894(83)90099-1

Experimental Parasitology

Volume 56, Issue 1, August 1983, Pages 89-92

https://doi.org/10.1016/0014-4894(83)90099-1 Get rights and content

Abstract

Both prostaglandin E₁ (PGE₁) and prostaglandin F_2α (PGF_2α) stimulate the glycogen phosphorylase (EC 2.4.1.1.) activity of Fasciola hepatica. Whole or sliced parasites were incubated with PGE₁ (2.8 × 10⁻⁷ and 2.8 × 10⁻⁵M) and PGF_2α(2.1 × 10⁻⁷ and 2.1 × 10⁻⁵M) and enzyme activity was measured in homogenates prepared immediately following the incubation. No substantially different effect was noted between the two assayed doses of prostaglandins. Prostaglandins appeared to be less effective in sliced parasites.

References (21)

C.H. Fiske et al.
Determination of inorganic phosphorus in serum and plasma
Journal of Biological Chemistry
(1925)
E. Gulbis et al.
Prostaglandin formation in bacteria
Prostaglandins
(1979)
O.N. Lowry et al.
Protein measurement with the Folin phenol reagent
Journal of Biological Chemistry
(1951)
F.A. Kuehl
Prostaglandins, cyclic nucleotides and cell function
Prostaglandins
(1974)
T.E. Mansour
Effect of serotonin on phenol oxidase from the liver fluke Fasciola hepatica and from other sources
Biochimica et Biophysica Acta
(1958)
T.E. Mansour et al.
The effect of serotonin (5-hydroxytryptamine) on the formation of adenosine 3′,5′-phosphate by tissue particles from the liver fluke, Fasciola hepatica
Journal of Biological Chemistry
(1960)
G. Markelonis et al.
Alterations of intracellular oxidative metabolism as stimuli evoking prostaglandin biosynthesis
Prostaglandins
(1975)
J.D. Smyth et al.
Egg-shell formation in Trematodes and Cestodes
Experimental Parasitology
(1959)
L.Z. Bito
The role of transport processes in the distribution and disposition of prostaglandins
A.H. Drummond et al.
Beta adrenergic regulation of glycogen phosphorylase activity and adenosine cyclic 3′,5′-monophosphate accumulation in control and desensitized C-6 astrocytoma cells
Molecular Pharmacology
(1977)

There are more references available in the full text version of this article.

Cited by (5)

Eicosanoids in parasites and parasitic infections
2000, Advances in Parasitology
Eicosanoids are lipid mediators with multiple functions in vertebrate tissues and invertebrate organisms. In this review the roles of eicosanoids — mostly prostaglandins (PGs), thromboxanes and leukotrienes — in parasite physiology and host-parasite interactions are discussed. PGs are present in the saliva of blood-sucking arthropods facilitating feeding by increasing local blood flow and prolonged attachment of ticks by immune suppression. Release of various eicosanoids has also been demonstrated for a number of protozoan and metazoan endoparasites. These substances appear to play a role in penetration, immune suppression, inflammation or modulation of haemostasis, enabling parasite invasion and establishment. Moreover, endogenous eicosanoids serve various functions in parasite metabolism and physiology. In many parasitic infections eicosanoids are involved in host pathology, e.g. granuloma formation, coagulapathy, secretory diarrhoea, or fever. Immune suppression by induction of PG release, in particular PGE2, by host defence cells appears to be a common feature of many parasitic infections and is thought to be important for parasite establishment. Contradictory results have been obtained for gastrointestinal nematode infections, which probably reflect the considerable differences between the various models employed. Although most of the available studies indicate an important role for eicosanoids in parasites and parasitic infections, our current knowledge is still fragmentary and more data are urgently needed.
Understanding chronic nematode infections: Evolutionary considerations, current hypotheses and the way forward
1992, International Journal for Parasitology
Intermediary metabolism in parasitic helminths
1987, International Journal for Parasitology
This review is a survey of progress in the field of helminth intermediary metabolism since ICOPA V. Data on the catabolism and synthesis of carbohydrates, lipids, amino acids, proteins, nucleotides and nucleic acids are presented and discussed in relation to previous work. The large number of references cited reflects a continued interest of parasite biochemists in this field, although many fundamental problems of helminth metabolism remain unsolved. Important areas for future research are identified and the potential application of new biochemical techniques, especially those in molecular biology, are emphasised.
Occurrence of prostaglandins and other eicosanoids in helminths and their role in host-parasite interaction - Review article
2001, Helminthologia
Energy metabolism and its regulation in the adult liver fluke Fasciola hepatica
1986, Parasitology

¹: Present address: Istituto di Fisiologia Veterinaria e Biochimica, Università degli Studi di Milano, Via Celoria, 10 I-20133 Milano, Italy.

View full text

Article preview

Experimental Parasitology

Abstract

References (21)

Determination of inorganic phosphorus in serum and plasma

Journal of Biological Chemistry

Prostaglandin formation in bacteria

Prostaglandins

Protein measurement with the Folin phenol reagent

Journal of Biological Chemistry

Prostaglandins, cyclic nucleotides and cell function

Prostaglandins

Effect of serotonin on phenol oxidase from the liver fluke Fasciola hepatica and from other sources

Biochimica et Biophysica Acta

The effect of serotonin (5-hydroxytryptamine) on the formation of adenosine 3′,5′-phosphate by tissue particles from the liver fluke, Fasciola hepatica

Journal of Biological Chemistry

Alterations of intracellular oxidative metabolism as stimuli evoking prostaglandin biosynthesis

Prostaglandins

Egg-shell formation in Trematodes and Cestodes

Experimental Parasitology

The role of transport processes in the distribution and disposition of prostaglandins

Beta adrenergic regulation of glycogen phosphorylase activity and adenosine cyclic 3′,5′-monophosphate accumulation in control and desensitized C-6 astrocytoma cells

Molecular Pharmacology

Cited by (5)

Eicosanoids in parasites and parasitic infections

Understanding chronic nematode infections: Evolutionary considerations, current hypotheses and the way forward

Intermediary metabolism in parasitic helminths

Occurrence of prostaglandins and other eicosanoids in helminths and their role in host-parasite interaction - Review article

Energy metabolism and its regulation in the adult liver fluke Fasciola hepatica