Regular articleMC29-immortalized clonal avian heart cell lines can partially differentiate in vitro☆
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Novel cell lines derived from adult human ventricular cardiomyocytes
2005, Journal of Molecular and Cellular CardiologyCitation Excerpt :Terminally differentiated primary cultures which may be maintained for several weeks, undergo morphological and functional changes over time [4,5], and yield a heterogeneous population of cells. Attempts to establish immortalized cardiomyocyte cell lines that can proliferate in culture include AT-1 cells, established from a mouse atrial tumor [6], MC29, a quail cell line from a rhabdomyosarcoma tumor [7], cell lines established from transgenic mice that express the SV40 T-ag [8–10], and by transformation of fetal cardiomyocytes with the SV40 oncogene [11–13]. These transformed cells suffer from numerous deficits including the lack of typical cardiomyocyte phenotype, limited capacity to be passaged, which compromises their applications to developmental and cellular studies, or de-differentiating on prolonged culture.
DSCAM: An endogenous promoter drives expression in the developing CNS and neural crest
2002, Biochemical and Biophysical Research CommunicationsCitation Excerpt :To identify functional elements of the endogenous DSCAM promoter for use in directing transgene expression in the mouse, we subcloned a fragment containing 1.8 kb of human genomic DNA immediately upstream of the DSCAM translational start site into the reporter vector pβgal-enhancer (Clontech) (see Fig. 1). The resulting transgenic construct, Pr1.8-βgal, was first tested for promoter activity in cell lines derived from several different tissue types, including COS-7 (ATCC), NIH/3T3 (ATCC), and a quail embryonic cardiac mesenchyme (MEQC) cell line [10] (see Methods). Transfection conditions were initially optimized for each cell line using the pβgal-control vector (Clontech) and a variety of transfection reagents, including Effectene (Qiagen), Superfect (Qiagen), and Lipofectamine (Gibco-BRL) (see Methods).
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Financial support was provided by the Centre National de la Recherche Scientifique and the Association Française contre la Myopathie.
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T.J is a recipient of a fellowship from the Fondation pour la Recherche Médicale.
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Present address: Ecole Normale Supérieure, Département de Biologie, 46, rue d'Ulm 75005 Paris, France.
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N.B is a recipient of a fellowship from the Fondation Singer-Polignac.