Action of papain, sugar, minoxidil, and glucan on excisional wounds in rats

doi:10.1016/0011-393X(95)85073-2

Current Therapeutic Research

Volume 56, Issue 12, December 1995, Pages 1285-1297

https://doi.org/10.1016/0011-393X(95)85073-2 Get rights and content

Abstract

Four topical drugs were studied with regard to their ability to promote healing of open wounds in 60 Wistar rats. Five study groups were defined. Four of the five groups received one of the following substances: papain, sugar, minoxidil, or glucan. The fifth group or control group received saline solution (NaCl, 0.9%). An experimental model with wound standardization (6 × 3.5 cm) was used. The raw area and new epithelium were quantified in each rat by taking photographs from which planimetry was performed. The closure of the first lesion occurred on the 49th postoperative day. Quantitative assessment of collagen deposition was performed using image analyzing software on slides stained with picrosirius and observed under polarized light. Epithelium formation was greatest in the minoxidil group, followed (in decreasing order) by the sugar, papain, control, and glucan groups. Significant differences in collagen deposition were observed among all the groups. The greatest amount of collagen was quantified in the glucan group, followed (in decreasing order) by the sugar, minoxidil, papain, and control groups. This data and possible mechanisms for the interference of each substance in wound healing are considered.

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Citation Excerpt :
This is a routine classical technique very suitable for the current purposes. It has been employed by other authors (Brenda et al., 1995; Sun et al., 1999; Sintov et al., 2000). Gel and cream preparations containing 0.8% (w/w) of papain 450 Wallerstein Papain Activity (W.P.A., Wallerstein) were produced as follows.
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Action of papain, sugar, minoxidil, and glucan on excisional wounds in rats

Abstract

Anal Biochem

J Invest Dermatol

Ann Emerg Med

Granulation tissue as a contractile organ

J Exp Med

The epithelium and the wound healing

Surg Gynecol Obstet

In vitro study of bacterial growth inhibition in concentrated sugar solutions: Microbiological basis for the use of sugar in treating infected wounds

Antimicrob Agents Chemother

A controlled model of moist wound healing: Comparison between semi-permeable film, antiseptics and sugar paste

J Exp Pathol

Successful treatment of chronically infected wounds with sugar paste

Eur J Clin Microbiol Infect Dis

Alopecia areata treated with topical minoxidil

J R Soc Med

Minoxidil: A review of its pharmacological properties and therapeutic use

Drugs