Regulation of the immune response: II. Concomitant idiotope-specific enhancement and suppression can result in a phenotypically normal response

doi:10.1016/0008-8749(86)90275-3

Cellular Immunology

Volume 98, Issue 1, March 1986, Pages 145-155

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Abstract

Idiotope-specific immunoenhancement or suppression was induced in C57BL/6 mice by the injection of physiological amounts (100 ng-10 μg) of monoclonal anti-idiotope antibody. As previously described, nanogram doses enhanced idiotope expression while a 10-μg dose of anti-idiotope antibody induced the activation of a population of Thy 1.2⁺, Lyt 1⁻, 2⁺ suppressors. Both positive and negative regulatory activities were confined to the non-μ, idiotope⁺ compartment of the plaque-forming cell response. Administration of intermediate doses of anti-idiotope antibody resulted in an immune state indistinguishable from that of naive mice. This apparently normal response was in fact the product of a simultaneous activation of balanced enhancing and suppressive activities. When treated with anti-Lyt 2 or -Lyt 1 and complement, spleen cell populations taken from such phenotypically “naive” mice revealed latent idiotope-specific immunoenhancement or suppression, demonstrating the components of a functional regulatory equilibrium.

References (19)

G. Kelsoe
J. Immunol. Methods
(1985)
A.L. Bothwell et al.
Cell
(1981)
J. Cerny et al.
Immunol. Today
(1984)
G. Kelsoe et al.
Immunol. Rev
(1980)
G. Kelsoe et al.
Eur. J. Immunol
(1981)
G. Kelsoe et al.
O. Makela et al.
Immunol. Rev
(1977)
R.S. Jack et al.
Eur. J. Immunol
(1978)
M. Reth et al.
Eur. J. Immunol
(1978)

There are more references available in the full text version of this article.

Cited by (2)

Anti-idiotypic antibodies as surrogate antigens for vaccinating against the cattle tick, Boophilus microplus
1995, Veterinary Parasitology
QU13, a monoclonal antibody (mAb) raised against midgut (GM) antigens from Boophilus microplus and shown to recognise antigens which protec cattle from tick challenge was used to immunise cattle and rabbits to produce anti-idiotypic antibodies (AIA). Polyclonal antisera against mAb QU13 were produced in rabbits and cattle. AIA were purified from these antisera by affinity chromatography procedures. These purified AIA were found to block mAb QU13 binding to GM in enzyme-linked immunosorbent assay (ELISA). AIA purified from bovine antiserum elicited an immune response in cattle to antigens extracted by detergent from the midgut of B. microplus (TXGM) after the fourth vaccination P = 0.06)_compared with the bovine immunoglobulin (Ig) control. The mean antibody level in the group of experimental cattle vaccinated with AIA purified from rabbit antisera was significantly higher (P < 0.03) than that of bovine Ig control cattle after the fourth vaccination and an anamnestic response (P < 0.11) occurred in the rabbit AIA vaccinated group of cattle when a single booster dose of 300 μg of TXGM was given after the first tick challenge. The positive control group of cattle vaccinated with TXGM were significantly protected (P < 0.05) against tick infestation after the booster dose of 300 μg of TXGM. The AIA vaccinated groups of cattle were not protected against challenge with 20 000 larval ticks either before or after the booster injection of 300 μg of TXGM.
Detection of human auto-anti-idiotypic antibodies (Ab2): II. Generation of ab2 in atopic patients undergoing allergen immunotherapy
1988, International Archives of Allergy and Immunology

^☆: This work was supported by a New Investigator Research Award (AI 20350) and, initially, by a European Molecular Biology Organization Long-Term Fellowship.

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Regulation of the immune response: II. Concomitant idiotope-specific enhancement and suppression can result in a phenotypically normal response☆

Abstract

J. Immunol. Methods

Cell

Immunol. Today

Immunol. Rev

Eur. J. Immunol

Immunol. Rev

Eur. J. Immunol

Eur. J. Immunol