HIV-1 proteinase is required for synthesis of pro-viral DNA

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Abstract

HIV-1 proteinase activity is thought to occur primarily post-integration by cleaving the viral Gag and Gag-Pol polyproteins. Its role in the pre-integration stages of viral replication, however, has not been studied in detail. Here we report that a synthetic peptide analogue, UK-88,947, which is a specific inhibitor of purified HIV-1 proteinase, inhibits the processing of the viral polyproteins in cultures of HIV-1 infected cells and prevents the formation of mature, infectious virions. Analysis of DNA from HIV-1 infected cells treated with UK-88,947 showed that viral DNA synthesis was inhibited when the compound was added to cultures one hour before infection. Similar results were obtained when AZT was used. Neither HIV-1 reverse transcriptase or the replication of FIV are inhibited by UK-88,947.

References (19)

  • M. Roberts et al.

    Biochem. Biophys. Res. Commun

    (1989)
  • D.E. Danley et al.

    Biochem. Biophys. Res. Commun

    (1989)
  • E. Matthes et al.

    Biochem. Biophys. Res. Commun

    (1987)
  • G. Gottlinger et al.
  • N.E. Kohl et al.
  • C. Peng et al.

    J. Virol

    (1989)
  • S. Seelmeier et al.
  • L.E. Henderson et al.
  • R. Lapatto et al.

    Nature

    (1989)
There are more references available in the full text version of this article.

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