P450_cam gene cloning and expression in $\text{Pseudomonas putida}$ and $\text{Escherichia coli}$

Abstract

The gene $\text{camC}$, which encodes the cytochrome P450 monoxygenase protein, was cloned into the shuttle vector pKT240 and recovered as the recombinant pKG201 with a 2.3 kb insert from the CAM plasmid in the $\text{Pst}$I site. The gene product is expressed constitutively in $\text{P. putida}$ and in $\text{E. coli}$ whereas the inverted insert clone lacks expression, indicating absence of an insert promoter.