Biochemical and Biophysical Research Communications
Cytidylate cyclase: The product isolated by the method of Cech and Ignarro is not cytidine 3′,5′-monophosphate
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Cited by (38)
Temporal and organ-specific detection of cNMPs including cUMP in the zebrafish
2015, Biochemical and Biophysical Research CommunicationsCitation Excerpt :cAMP and cGMP are well-established second messengers that regulate multiple physiological functions [1]. The existence of additional cyclic nucleotides, e.g. cCMP and cUMP, was claimed many years ago [2], but there were several setbacks in the beginning due to insufficient specificity and sensitivity of the available detection methods [3–5]. In recent studies, cCMP and cUMP have been detected in several cell lines using HPLC-MS/MS [6].
From canonical to non-canonical cyclic nucleotides as second messengers: Pharmacological implications
2015, Pharmacology and TherapeuticsCitation Excerpt :Unfortunately, the cyclic pyrimidine nucleotide field took another serious setback by claims published in a high-profile journal that mammalian cells express a specific CC (Cech & Ignarro, 1977). This claim turned out to be a methodological artifact (Gaion & Krishna, 1979), and a specific mammalian CC was never identified at the molecular level (Seifert, in press). Moreover, cCMP antibodies were much less specific than assumed, raising serious doubts about the validity of immunological studies on cCMP levels in biological systems (Anderson, 1982).
cCMP and cUMP: Emerging second messengers
2015, Trends in Biochemical SciencesCitation Excerpt :In 1971, the Stock group identified a cUMP-degrading PDE in fat cells [2], but these findings were not followed up on. Development of the young field was compromised by claims of the existence of a specific mammalian cytidylyl cyclase (CC) [3] (see Glossary) that turned out to be a methodological artifact [4]. Antibodies against cCMP showed cross-reactivities with other nucleoside 3′,5′-cyclic monophosphates (cNMPs) [5], and early MS methods were insufficiently sensitive and specific to unequivocally quantitate and identify cCMP and cUMP [6].
PDE7A1 hydrolyzes cCMP
2014, FEBS Letters
- 1
Abbreviations: Cyclic CMP, cytidine 3′,5′-monophosphate.