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Studies on kinase-controlled state transitions in Photosystem II and b6f mutants from Chlamydomonas reinhardtii which lack quinone-binding proteins

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Abstract

We have studied kinase-dependent state transitions in vivo using photosynthetic mutants from the green alga Chlamydomonas reinhardtii lacking in quinone-binding proteins. The aim of our study was to identify proteins involved in the plastoquinone-dependent activation of the LHC-kinase. Whereas mutants totally devoid of the quinone-binding subunits D1 and D2 of Photosystem II showed unaltered state transitions, mutants lacking the b6f complexes were incapable of state transitions. These mutants were blocked in State 1, which is indicative of either the absence of the LHC-kinase responsible for the regulation, or of the loss of a component responsible for the activation of this enzyme. These two hypotheses are discussed in light of (i) the patterns of phosphorylation of the thylakoid membrane proteins observed in the b6f mutants and (ii) the characteristics of the kinase activities recovered from their thylakoids.

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