The Bromoviruses

https://doi.org/10.1016/S0065-3527(08)60660-0Get rights and content

Publisher Summary

Brome mosaic virus (BMV), broad bean mottle virus (BBMV), and cowpea chlorotic mottle virus (CCMV) are collectively known as the bromoviruses. Although these plant viruses differ widely in their host ranges, they are all spherical with anhydrous diameters of about 25 nm; they all contain about a million daltons of RNA and 180 protein subunits weighing about 20,000 daltons each. The bromoviruses are particularly suited to the studies of virus structure and of plant virus replication. They are stable, easily isolated in gram quantities, and they are the only spherical viruses that can be easily and efficiently reassembled from protein and RNA. Their genomes, which consist of three independently encapsidated RNA components, are amenable to crude genetic analysis. Brome mosaic virus RNA is an efficient messenger in a wheat embryo protein synthesizing system. Cowpea chlorotic mottle virus infects the tobacco protoplasts efficiently and synchronously. The basic tools are now available for studying the bromovirus replication at the molecular level. This chapter approaches the bromoviruses as a molecular biologist. After briefly introducing the virus, it proceeds from the simplest molecular aspects of the virus, the structure of its protein and RNA, to the virus itself and finally, to the more complex biological properties of the virus.

References (281)

  • H.O. Agrawal et al.

    Virology

    (1972)
  • J.W. Anderegg et al.

    Biophys. J.

    (1963)
  • A.I. Aronson et al.

    Virology

    (1962)
  • J.B. Bancroft

    Advan. Virus Res.

    (1970)
  • J.B. Bancroft

    Virology

    (1971)
  • J.B. Bancroft et al.

    Virology

    (1967)
  • J.B. Bancroft et al.

    Virology

    (1967)
  • J.B. Bancroft et al.

    Virology

    (1968)
  • J.B. Bancroft et al.

    Virology

    (1968)
  • J.B. Bancroft et al.

    Virology

    (1969)
  • J.B. Bancroft et al.

    Virology

    (1969)
  • J.B. Bancroft et al.

    Virology

    (1971)
  • S.V. Beer et al.

    Virology

    (1970)
  • L.E. Bockstahler et al.

    Biophys. J.

    (1962)
  • L.E. Bockstahler et al.

    J. Mol. Biol.

    (1965)
  • L.E. Bockstahler et al.

    Virology

    (1965)
  • H. Boedtker

    J. Mol. Biol.

    (1968)
  • H. Boedtker

    Biochim. Biophys. Acta

    (1971)
  • F. Bonhoeffer et al.

    Biochem. Biophys. Res. Commun.

    (1960)
  • M.K. Brakke

    Virology

    (1958)
  • M.K. Brakke

    Virology

    (1963)
  • M.K. Brakke

    Virology

    (1971)
  • M.K. Brakke et al.

    Virology

    (1970)
  • R. Burroughs et al.

    Virology

    (1966)
  • P.J.G. Butler et al.

    J. Mol. Biol.

    (1972)
  • J. Chidlow et al.

    Virology

    (1971)
  • M.F. Clark et al.

    Virology

    (1971)
  • E.M. Davison et al.

    Virology

    (1969)
  • W.O. Dawson et al.

    Virology

    (1972)
  • G.A. de Zoeten et al.

    Virology

    (1967)
  • G.A. de Zoeten et al.

    Virology

    (1967)
  • A. Dingjan-Versteegh et al.

    Virology

    (1972)
  • J.A. Dodds et al.

    Virology

    (1972)
  • A.K. Dunker et al.

    J. Biol. Chem.

    (1969)
  • A.C.H. Durham

    J. Mol. Biol.

    (1972)
  • J.T. Finch et al.

    J. Mol. Biol.

    (1967)
  • J.T. Finch et al.

    J. Mol. Biol.

    (1967)
  • E. Fowlks et al.

    Virology

    (1970)
  • S.A. Ghabrial et al.

    Virology

    (1973)
  • A.J. Gibbs

    Advan. Virus Res.

    (1969)
  • D.G. Glitz et al.

    Biochim. Biophys. Acta

    (1971)
  • A. Hadidi et al.

    Virology

    (1973)
  • R.I. Hamilton

    Virology

    (1961)
  • V. Hariharasubramanian et al.

    Virology

    (1973)
  • B.D. Harrison et al.

    Virology

    (1971)
  • E. Hiebert et al.

    Virology

    (1969)
  • E. Hiebert et al.

    Virology

    (1968)
  • J.H. Hill et al.

    Virology

    (1972)
  • G.J. Hills et al.

    J. Ultrastruct. Res.

    (1968)
  • A. Hirai et al.

    Virology

    (1968)
  • Cited by (93)

    • Revisiting the genome packaging in viruses with lessons from the "Giants"

      2014, Virology
      Citation Excerpt :

      The sgRNA make up secondary RNA molecules necessary for replication as well as packaging. The reason for the packaging of a segmented genome is not well understood (Lane, 1974; Mossop and Francki, 1979; Rao, 2006; Simon et al., 2004). Like plant viruses, influenza virus and flock house virus (FHV) genomes are also segmented.

    • The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous

      2014, Journal of Molecular Biology
      Citation Excerpt :

      BMV is a multipartite positive-strand RNA virus that can infect monocot and dicot plants. BMV infection is locally confined in two Chenopodium species but can spread systemically in Chenopodium quinoa, the geranium tobacco, Nicotiana benthamiana, and barley and wheat [3–5]. The outcome of the viral infection is due to the compatibility of the interactions between plant pathogen sensors and the pathogen molecules [6].

    • Virus Diseases of Peas, Beans, and Faba Bean in the Mediterranean Region

      2012, Advances in Virus Research
      Citation Excerpt :

      For a long time, BBMV attracted academic interest because of its structural characteristics; it reaches high concentration in infected cells and is an easy virus to purify. The virus was successfully purified as described by Lane (1974). A purified preparation of the virus has an A260:A280 ratio of 5.4.

    View all citing articles on Scopus
    1

    Present address: Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska.

    View full text