Basic scienceCan mycoplasma-mediated oncogenesis be responsible for formation of conventional renal cell carcinoma?
Section snippets
Subjects
Tissue samples from 33 patients with cRCC who had undergone radical nephrectomy were studied (mean age, 62 years; male/female ratio 2.3:1). The tumors were histologically classified according to the criteria described by Störkel et al.8 The TNM 1997 classification system was used for tumor staging, and the tumors were graded according to Fuhrman nuclear grading system.9, 10 Patients with T1N0M0, T2N0M0, and T3aN0M0 tumors were accepted as having localized disease; patients with all other stages
Results
Nested PCR was successfully done in all DNA samples isolated from normal kidney, RIN, and tumor tissues of patients with cRCC. Figure 1 shows the gel image of the nested PCR products, including mycoplasma-positive controls and patients. Amplified control tissues and samples had between 200 and 350-bp bands. Mycoplasma sp. DNA was detected in 12 (36%) of 33 samples of normal kidney, 22 (67%) of 33 samples of RIN, and 27 (82%) of 33 tumor samples. In 6 of the 33 patients with RCC, no Mycoplasma
Comment
Mycoplasma sp. exist widely in nature. Mycoplasma infections are widespread in cell culture studies and are a problem in laboratory studies. Mycoplasma sp. can cause chromosomal abnormalities in many cell types, and these cells can gain an oncogenic potential in long-lasting infections (6 weeks or more).6, 7, 13, 14 Mycoplasma sp. rDNA was detected in 56% of gastric carcinoma tissues, 28% of chronic superficial gastritis, 30% of gastric ulcer, 37% of intestinal metaplasia, 55% of colon cancer,
Conclusions
Our results suggest an association between Mycoplasma sp. infection and cRCC. Confirmation of our results by other investigators may help to highlight the role of mycoplasma in the development of cancer.
References (25)
- et al.
Tubular dysplasia and carcinoma in situprecursors of renal cell carcinoma
Urology
(1999) - et al.
A polymerase reaction based method for detecting Mycoplasma Acholeplasma contaminants in cell culture
J Microbiol Method
(2000) - et al.
Does Mycoplasma sp. play role in small cell lung cancer?
Lung Cancer
(2004) - et al.
Microsatellite instability in gastric intestinal metaplasia in patients with and without gastric cancer
Am J Pathol
(2000) - et al.
Translational infidelity and human cancerrole of the PTI-1 oncogene
Int J Biochem Cell Biol
(1999) - et al.
Mycoplasmal infections alter gene expression in cultured human prostatic and cervical epithelial cells
FEMS Immunol Med Microbiol
(2000) - et al.
DNA mismatch repair genes in renal cell carcinoma
J Urol
(2003) - et al.
Renal cell carcinomanew prognostic factors?
Curr Opin Urol
(2003) - et al.
Premalignant lesions in the kidney
Sci World J
(2001) - et al.
Proliferative activity, angiogenesis and nuclear morphometry in renal cell carcinoma
Int J Urol
(2001)
Premalignant lesions of the kidney share the same genetics changes as conventional renal cell carcinoma
World J Urol
Mycoplasmas and oncogenesispersistent infections and multistage malignant transformation
Proc Natl Acad Sci USA
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