Success in the isolation and axenic culture of Anaptychia ciliaris (Physciaceae, Lecanoromycetes) mycobiont

M. Carmen Molina; Pradeep K. Divakar; Natalia González

doi:10.1016/j.myc.2014.10.003

Full paper

Success in the isolation and axenic culture of Anaptychia ciliaris (Physciaceae, Lecanoromycetes) mycobiont

M. Carmen Molina , Pradeep K. Divakar, Natalia González

Author information

Keywords: Aposymbiotic cultures, Development, Lichen, Morphogenetic differences, Ontogeny

JOURNAL OPEN ACCESS

2014 Volume 56 Issue 4 Pages 351-358

DOI https://doi.org/10.1016/j.myc.2014.10.003

Details

Abstract

The aim of this study was to evaluate the optimum conditions for culturing Anaptychia ciliaris mycobionts in vitro and analyzing their ontogenetic development while in culture. After the testing of two germination and four development media, results of germination and growth were evaluated. We here recommend the use of basal medium without additional carbon source for germination process. Mycobiont growth was possible in all organic media tested. The maximum mycobiont growth was observed in Lilly and Barnet medium enriched with 3% glucose.

Corresponding author

Register with J-STAGE for free!