Placental expression of SCO-spondin during mouse and human development
Section snippets
Results and discussion
SCO-spondin transcripts were detected at the first stages of mouse (7 days post-coı̈tum, 7 dpc) and human (6th week of gestation) placentation (lines 3 and 7 of Fig. 1) and were still present until placental senescence and parturition (lines 6 and 10 of Fig. 1 and data not shown). The SCO-spondin was a new member of the thrombospondin super-family to be expressed in placental structures during mammalian development. Nevertheless, it was the first time that this chorio-allantoic placental
Methods
Placental tissues and amniotic fluid were (i) frozen at −80 °C for RT-PCR and Western-blot experiments and (ii) placed in molds with embedding medium and frozen on the surface of dry ice for immunohistochemistry assays. Sectioning and immunohistochemistry were performed as already described (Blanchon et al., 2001) using three primary rabbit specific epitop antibodies against bovine Reissner's fiber isolated from the central canal (Meiniel et al., 1996), a peptide deduced from the mouse
Acknowledgements
Nicolas Gonçalves-Mendes and Loı̈c Blanchon are supported by a grant from the Ministère de l'Education de la Recherche et de la Technologie (MERT).
References (22)
- et al.
Co-localization of KLF6 and KLF4 with pregnancy-specific glycoproteins during human placenta development
Mech. Dev.
(2001) - et al.
Characterization of ADAMTS14, a novel member of the ADAMTS metalloproteinase family
Biochim. Biophys. Acta
(2001) - et al.
Complex expression pattern of the SCO-spondin gene in the bovine subcommissural organ: toward an explanation for Reissner's fiber complexity?
Mol. Brain Res.
(1998) - et al.
Genes, development and evolution of the placenta
Placenta
(2003) - et al.
A new FACIT of the collagen family: COL21A1
Fed. Eur. Biochem. Soc. Lett.
(2001) - et al.
Mouse SCO-spondin, a gene of the TSR superfamily expressed in the brain
Gene
(2003) - et al.
Expression of ADAMTS1 during murine development
Mech. Dev.
(2002) - et al.
The proneural genes NEUROD1 and NEUROD2 are expressed during human trophoblast invasion
Mech. Dev.
(2002) - et al.
The thrombospondin type 1 repeat (TSR) superfamily: diverse proteins with related roles in neuronal development
Dev. Dyn.
(2000) - et al.
A role for the cytoskeleton-associated protein palladin in neurite outgrowth
Mol. Biol. Cell
(2001)