Regeneration of meniscus cartilage in a knee treated with percutaneously implanted autologous mesenchymal stem cells☆
Section snippets
Autologous platelet approaches
The platelet augmentation approach is based on the concept that platelets contain key growth factors such as platelet derived growth factors (PDGF’s), transforming growth factors (TGF’s), fibroblast growth factors (FGF’s), and various forms of interleukins (IL) [6]. In response to tissue injury, a complex cascade of cellular and noncellular signals triggers platelet receptors, resulting in expulsion of these growth factors within the site of injury. This process is known as degranulation and
Stem cells and regenerative medicine
Embryonic stem cells have been in the popular media for many years and have shown promise scientifically while creating significant controversy. As a result, many researchers have focused on adult stem cells, or stem cells isolated from adult humans that can be transplanted into damaged tissue. Adult stem cells consist of three main types: CD34+ heme progenitors destined to become blood components, endodermal stem cells used to replace endodermal linings such as endometrium, and mesenchymal
Growth factors in regenerative medicine
Recombinant human growth factors have just begun to be used in modern clinical practice. These are proteins in the cytokine family that have the ability to promote growth of certain tissues. The first of these to be available in spinal surgery practices is the bone morphogenic protein (BMP) family. Both BMP-2 and BMP-7 are currently being used to promote fusion [53], [54]. Animal trials with these growth factors may show some promise in repair of peripheral joints [55]. In addition, FGF-2 has
Results
The pre and post-procedure MRI analysis demonstrated an increase in meniscus volume that was more than the standard deviation of the serial three ROI measurements. At three month follow-up, modified VAS scores decreased from 3.33 to 0.13.
Conclusion
This case report shows MRI evidence of increased meniscus volume. While there has been evidence from animal models of cartilage regeneration using MSC’s, this is the first case report of an increase in meniscus size in a human subject (of which we are aware). While the patient reported clinical response could have been due to the dexamethasone injection provided post transplant procedure, the levels injected (10 ng/ml) were approximately one million times lower than those used clinically
References (56)
Recruitment of mesenchymal stem cells and expression of TGF-beta 1 and VEGF in the early stage of shock wave-promoted bone regeneration of segmental defect in rats
J Orthop Res
(2004)- et al.
Effects of calcium and thrombin on growth factor release from platelet concentrates: kinetics and regulation of endothelial cell proliferation
Biomaterials
(2004) Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM
Cytotherapy
(2006)The biology of hematopoietic stem cells
Arch Med Res
(2003)The manipulated mesenchymal stem cells in regenerated skeletal tissues
Cell Transplant
(1998)- et al.
Genetically modified CD34+ cells do not contribute to the mesenchymal compartment after autologous transplantation in the baboon
Cytotherapy
(2005) An arthroscopic treatment regimen for osteoarthritis of the knee
Arthroscopy
(2007)- et al.
TGF-beta and osteoarthritis
Osteoarthritis Cartilage
(2007) Osteochondrogenic potential of marrow mesenchymal progenitor cells exposed to TGF-beta 1 or PDGF-BB as assayed in vivo and in vitro
J Bone Miner Res
(1996)Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications
J Cell Physiol
(2005)
Capture and expansion of bone marrow-derived mesenchymal progenitor cells with a transforming growth factor-beta1-von Willebrand’s factor fusion protein for retrovirus-mediated delivery of coagulation factor IX
Hum Gene Ther
Bone induction by osteogenin and bone morphogenetic proteins
Biomaterials
Platelet-rich plasmas: growth factor content and roles in wound healing
J Dent Res
Autologous platelets as a source of proteins for healing and tissue regeneration
Thromb Haemostasis
Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell-therapy approaches: further insights in the search for a fetal calf serum substitute
J Cell Physiol
Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells
Transfusion
Treatment of chronic elbow tendinosis with buffered platelet-rich plasma
Am J Sport Med
Platelet concentrate injection improves Achilles tendon repair in rats
Acta Orthop Scand
Articular cartilage: degeneration and osteoarthritis, repair, regeneration, and transplantation
Instr Course Lect
Mesenchymal stem cells
J Orthop Res
Mechanobiology of skeletal regeneration
Clin Orthop Relat Res
Autologous mesenchymal progenitor cells in articular cartilage repair
Clin Orthop Relat Res
Mesenchymal stem cells in osteoarthritis
Curr Opin Rheumatol
Mesenchymal stem cell therapy to rebuild cartilage
Trends Mol Med
Stem cell therapy in a caprine model of osteoarthritis
Arthritis Rheum
Regenerative medicine through mesenchymal stem cells for bone and cartilage repair
Curr Opin Invest Drugs
Current strategies for articular cartilage repair
Eur Cell Mater
Cited by (113)
Use of bone marrow derived mesenchymal stem cells for the treatment of osteoarthritis: A retrospective long-term follow-up study: BMAC for knee osteoarthritis
2023, Journal of Clinical Orthopaedics and TraumaImmunoregulatory properties of mesenchymal stem cells and their application in immunotherapy
2020, Mesenchymal Stem Cells in Human Health and DiseasesIsolation, expansion and application of human mesenchymal stem cells
2019, Comprehensive BiotechnologyImmunity-and-matrix-regulatory cells enhance cartilage regeneration for meniscus injuries: a phase I dose-escalation trial
2023, Signal Transduction and Targeted TherapyTreatment strategies for meniscal lesions: from past to prospective therapeutics
2022, Regenerative Medicine
- ☆
Financial Support was from Regenerative Sciences, INC 11080 Circle Point Rd Buliding 2, Suite 140. Westminster, CO 80020.
- 1
Tel.: +303 429 6448; fax: +303 429 6763.
- 2
Tel.: +970 476 1100.