Transplantation infectionA novel model to study bacterial adherence to the transplanted airway: Inhibition of Burkholderia cepacia adherence to human airway by dextran and xylitol
Section snippets
Bacteria and culture conditions
All Bcc isolates used in this study, except for ATCC 25416, were isolated from the sputa of patients with CF. We isolated BC7 (genomovar IIIa), which belongs to the ET12 clonal lineage and has been described previously,14, 23 from a Toronto patient with CF. C5393 (genomovar II), FC473 (genomovar IV), and CEP040 (genomovar V) are Bcc research panel isolates24 and were kindly provided by Dr. Mahenthiralingam (Cardiff School of Biosciences, Cardiff University, UK). ATCC 25416 (genomovar I) is an
Morphology of airway explants and immunostaining of mucins
Airway explants maintained in BEGM at an air-liquid interface for 24 hours showed normal pseudostratified epithelium composed of columnar ciliated cells, goblet cells, and basal cells (Figure 1A, arrows). A thick basement membrane, characteristic of proximal airways, was observed below the epithelium. Beneath the basement membrane was the lamina propria with richly vascularized connective tissue containing monocytes. The apical surface was covered with a mucuslike layer (Figure 1A, asterisks).
Discussion
We have demonstrated that airway explants obtained at the time of lung transplantation can be used to study bacterial adherence. This system models in part the in vivo situation, because it represents the ischemic, cold, stored human airway segments consisting of well-differentiated respiratory epithelia with mucus-secreting and ciliated cells and a protective coat of mucus covering the apical surface of intact epithelia. The 5 tested Bcc genomovars exhibited binding to the explants, and the
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