Essential oil of Ayapana triplinervis from Reunion Island: A good natural source of thymohydroquinone dimethyl ether

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Abstract

Three specimens of Ayapana triplinervis (Vahl) R.M. King & H. Rob from Reunion Island (Indian Ocean) collected at two distant locations (North of the island; samples 1 and 2, South of the island; sample 3), in different growth phases (flowering; samples 1 and 3, vegetative; sample 2) were investigated for their leaf essential oil composition. This study reports the chemical character of this species on the island and investigates the relationship between essential oil composition, developmental stage and geographic location. Analysis by GC–FID and GC–MS enabled us to identify and quantify a total of 39 constituents accounting for 97.1–98.0% of the oils. The three essential oil samples, all obtained by hydrodistillation, showed a high percentage of the aromatic compound thymohydroquinone dimethyl ether (89.9–92.8%). All other minor components remained more or less unchanged both qualitatively and quantitatively with respect to the stage of growth. On the contrary, variations were observed with geographic distribution. The geographical variation of the chemical composition of the volatile oil of A. triplinervis from several sites in the world is also briefly discussed.

Introduction

Ayapana triplinervis (Vahl) R.M. King & H. Robinson (syn.: Eupatorium ayapana Vent; Eupatorium triplinerve Vahl) belongs to the Asteraceae family and is commonly known as Ayapana in Hindi. It is also known by several other names, such as Ayapana tea in England, Ayapan, Bishallakarani in Bangladesh, Aiapana, Iapana, Japana, Japana-branca, Japana-roxa, Erva-de-cobra, Erva-santa in Brazil, Aiapana, Diapana, Thé de l'Amazone in French Guiana, Sekrepatoewiwiri in Surinam and Herbe à Thé, Herbe vulnéraire in French West Indies. This plant growing up to 1 m high is an ornamental erect perennial herb and semi-woody at base. The leaves (4.5–10.5 cm long and 0.8–1.7 cm wide) are aromatic, smooth, simple, opposite, sub-sessile, 3-nerved, acuminate, glabrous and lanceolate. The stems are reddish brown. The many flowering heads are each 6–13 mm long and bear about 40 pink flowers. A. triplinervis is native to South America and can be found in the Amazon region of Brazil, Ecuador, Peru, the three Guianas, Puerto Rico, Hawaii but is also well represented in other countries such as India, Vietnam and the Mascarene Islands (Reunion, Mauritius, Rodrigues). Since it is widely used in folk medicine (e.g. Agapito and Sung, 2003, Beckstrom-Sternberg et al., 1994, Bose and Roy, 1936, Brack Egg, 1999, Grenand et al., 1987, Gurib-Fakim and Guého, 1995, Manfred, 1947, Mors et al., 2000, Trang et al., 1993a, Van den Berg, 1984), the plant has been extensively investigated for its biological and pharmacological properties (e.g. Bose et al., 2007, Chaurasia and Kher, 1978, Facknath, 1999, Garg and Nakhare, 1993, Gupta et al., 2002, Jelager et al., 1998, Kokate et al., 1971, Verpoorte and Dihal, 1987, Yadava and Saini, 1990). However, till now the information about its chemical composition has remained poor. According to previous phytochemical studies (Bose and Roy, 1936, Chaturvedi and Mulchandani, 1989, Natarajan and Natarajan, 1979, Späth et al., 1937, Trang et al., 1992, Trang et al., 1993a, Trang et al., 1993b), the most characteristic secondary metabolites are coumarins (Fig. 1). A total of seven coumarins known under the trivial name ayapanin (or herniarin) (1), ayapin (2), daphnetin (3), daphnetin dimethyl ether (4), daphnetin-7-methyl ether (5), hydrangetin (6) and umbelliferone (7) have been characterized in the plant. Coumarins are considered to be components of the general defense response of plants to abiotic and biotic stresses and it has been proved that various substituted coumarins exhibit antimicrobial or anti-inflammatory activity and act as inhibitors of numerous enzyme systems (Murray et al., 1982). This may explain why A. triplinervis rich in coumarins, is used in herbal medicine. Concerning more specifically volatile compounds from A. triplinervis, few investigations (four references, all using the synonym Eupatorium triplinerve to designate the plant) exclusively devoted to essential oil samples of Brazilian, Indian and Vietnamese origins have been carried out (Table 1). According to this literature survey, it also appears that up to now the chemical composition of the volatile oils of A. triplinervis from Reunion Island has not been investigated. Yet this plant is locally really appreciated for its healing virtues in particular its digestive properties (Gurib-Fakim and Guého, 1995). Therefore, the present work concerns detailed GC and GC–MS examination of the leaf essential oil of three A. triplinervis specimens from Reunion Island, growing in two distinct locations (North of the island; samples 1 and 2, South of the island; sample 3), at two developmental stages (flowering; samples 1 and 3, vegetative; sample 2). The objective of our study was to (1) observe the homogeneity of the composition or, conversely, to evidence a chemical variability among A. triplinervis specimens on Reunion Island, and (2) compare the oil composition of A. triplinervis from Reunion Island with those of oils produced by the same species grown in different geographical regions of the world. This study is a part of the ongoing APLAMEDOM (Association for Medicinal and Aromatic plants of Reunion Island) program which aims at conserving biodiversity on Reunion Island through the valorisation of aromatic and medicinal plants. By reinforcing links between scientific information and ethnobotanical knowledge and by enabling systematic cultivation and mass propagation of aromatic and medicinal plants, the objective of this program is to develop a market of natural products on the island.

Section snippets

Plant material

Three samples of A. triplinervis (Vahl) R.M. King & H. Rob were gathered at two different stage of development (flowering and vegetative) in two different localities of Reunion Island (Table 2). Voucher specimens were identified and deposited at the Herbarium of the University of Reunion Island (REU).

Essential oil isolation

Fresh leaves (300 g) of the three samples were hydrodistilled in a Clevenger-type apparatus for 3 h. The oils were taken up in dichloromethane, dried over anhydrous sodium sulphate and kept at 4 °C.

Oil yield

Several studies on aromatic plants have shown that oil yield may be affected by environmental conditions such as moisture level and may fluctuate daily or seasonally as well (Hay and Waterman, 1993). However, in this study, the three oil yields (0.28–0.29%, calculated from fresh material) were quite similar. Thus, by this first approach, it seems that oil yields of A. triplinervis from Reunion Island may neither be affected by the site of development (North of the island; sample 1, South of the

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