Biochimica et Biophysica Acta (BBA) - Bioenergetics
Volume 1797, Issue 9, September 2010, Pages 1638-1646
Role of specific residues in coenzyme binding, charge–transfer complex formation, and catalysis in Anabaena ferredoxin NADP+-reductase
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Abbreviations
FNR
ferredoxin-NADP+ reductase
FNRox
FNR in the fully oxidized state
FNRrd
FNR in the hydroquinone (fully reduced) state
2′-P
2′-phosphate group of NADP+/H
ET
electron transfer
HT
hydride transfer
WT
wild-type
MC
Michaelis complex
CTC
charge–transfer complex
CTC-1
FNRox-NADPH CTC
CTC-2
FNRrd-NADP+ CTC
2′-P-AMP
2′-P-AMP moiety of NADP+/H
kA → B, kB → C
apparent rate constants obtained by global analysis of spectral kinetic data
kobsHT, kobsHT-1, kobsDT, kobsDT-1
observed conversion HT and DT rate constants for the forward and reverse reactions
kHT, kHT-1
hydride transfer first-order rate constants for the forward and reverse reactions, respectively
kDT, kDT-1
deuteride transfer first-order rate constants for the forward and reverse reactions, respectively
KdNADPH, KdNADP+
dissociation constants for the intermediate complexes in the reduction and reoxidation of FNR, respectively: FNR4, T155G/A160T/L263P/Y303S FNR variant
KIE
kinetic isotopic effect
Keywords
Ferredoxin-NADP+ reductase
Hydride transfer
Charge–transfer complex
Site-directed mutagenesis
Kinetic isotopic effect
Isoalloxazine–nicotinamide interactions
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